CRISPR-U™ Gene Knockout Cell Line Strategy

SMARCD1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human SMARCD1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SMARCD1
Gene id 6602
Organism Homo sapiens
Official full symbol SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1
Gene type protein-coding
Also known as BAF60A, CRACD1, CSS11, Rsc6p
Summary The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. Two transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 12
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
SMARCD1-202 ENST00000394963.9 3283 515aa Protein coding CCDS8797 Q96GM5-1 NM_003076.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
SMARCD1-201 ENST00000381513.8 3237 474aa Protein coding CCDS8798 Q96GM5-2 - TSL:1, GENCODE basic,
SMARCD1-205 ENST00000548573.5 2095 313aa Protein coding - F8VRQ4 - TSL:5, GENCODE basic,
SMARCD1-209 ENST00000550477.5 911 289aa Protein coding - F8VUB0 - CDS 3' incomplete, TSL:3,
SMARCD1-212 ENST00000551966.5 881 278aa Protein coding - F8VZ70 - CDS 3' incomplete, TSL:5,
SMARCD1-211 ENST00000551497.1 536 113aa Protein coding - F8VW95 - CDS 3' incomplete, TSL:4,
SMARCD1-206 ENST00000549274.1 611 115aa Nonsense mediated decay - H0YHV1 - CDS 5' incomplete, TSL:3,
SMARCD1-207 ENST00000549526.1 401 No protein Processed transcript - - - TSL:5,
SMARCD1-203 ENST00000547247.5 1584 No protein Retained intron - - - TSL:1,
SMARCD1-210 ENST00000551352.1 621 No protein Retained intron - - - TSL:2,
SMARCD1-204 ENST00000547637.1 587 No protein Retained intron - - - TSL:2,
SMARCD1-208 ENST00000550280.1 567 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SMARCD1
This KO Strategy loading
Red Cotton™ Notes Gene SMARCD1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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