CRISPR-U™ Gene Knockout Cell Line
Strategy
SMARCD1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SMARCD1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SMARCD1 |
| Gene id | 6602 |
| Organism | Homo sapiens |
| Official full symbol | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1 |
| Gene type | protein-coding |
| Also known as | BAF60A, CRACD1, CSS11, Rsc6p |
| Summary | The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. Two transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SMARCD1-202 | ENST00000394963.9 | 3283 | 515aa | Protein coding | CCDS8797 | Q96GM5-1 | NM_003076.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| SMARCD1-201 | ENST00000381513.8 | 3237 | 474aa | Protein coding | CCDS8798 | Q96GM5-2 | - | TSL:1, GENCODE basic, |
| SMARCD1-205 | ENST00000548573.5 | 2095 | 313aa | Protein coding | - | F8VRQ4 | - | TSL:5, GENCODE basic, |
| SMARCD1-209 | ENST00000550477.5 | 911 | 289aa | Protein coding | - | F8VUB0 | - | CDS 3' incomplete, TSL:3, |
| SMARCD1-212 | ENST00000551966.5 | 881 | 278aa | Protein coding | - | F8VZ70 | - | CDS 3' incomplete, TSL:5, |
| SMARCD1-211 | ENST00000551497.1 | 536 | 113aa | Protein coding | - | F8VW95 | - | CDS 3' incomplete, TSL:4, |
| SMARCD1-206 | ENST00000549274.1 | 611 | 115aa | Nonsense mediated decay | - | H0YHV1 | - | CDS 5' incomplete, TSL:3, |
| SMARCD1-207 | ENST00000549526.1 | 401 | No protein | Processed transcript | - | - | - | TSL:5, |
| SMARCD1-203 | ENST00000547247.5 | 1584 | No protein | Retained intron | - | - | - | TSL:1, |
| SMARCD1-210 | ENST00000551352.1 | 621 | No protein | Retained intron | - | - | - | TSL:2, |
| SMARCD1-204 | ENST00000547637.1 | 587 | No protein | Retained intron | - | - | - | TSL:2, |
| SMARCD1-208 | ENST00000550280.1 | 567 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SMARCD1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SMARCD1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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