CRISPR-U™ Gene Knockout Cell Line
Strategy
SMARCB1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SMARCB1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SMARCB1 |
| Gene id | 6598 |
| Organism | Homo sapiens |
| Official full symbol | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 |
| Gene type | protein-coding |
| Also known as | BAF47, CSS3, INI1, MRD15, PPP1R144, RDT, RTPS1, SNF5, SNF5L1, SWNTS1, Sfh1p, Snr1, hSNFS |
| Summary | The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Alternatively spliced transcript variants have been found for this gene. |
| Genomic regions | Chromosome 22 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SMARCB1-213 | ENST00000644036.2 | 5191 | 385aa | Protein coding | CCDS13817 | Q12824-1 | NM_003073.5 | GENCODE basic, APPRIS ALT1, MANE Select v0.92, |
| SMARCB1-204 | ENST00000407422.8 | 1730 | 376aa | Protein coding | CCDS46671 | Q12824-2 | - | TSL:1, GENCODE basic, APPRIS P4, |
| SMARCB1-202 | ENST00000344921.11 | 1717 | 394aa | Protein coding | CCDS82699 | G5E975 | - | TSL:2, GENCODE basic, |
| SMARCB1-201 | ENST00000263121.12 | 1500 | 339aa | Protein coding | - | A0A0G2JRV3 | - | TSL:1, GENCODE basic, |
| SMARCB1-205 | ENST00000417137.6 | 889 | 248aa | Protein coding | - | C9JTA6 | - | CDS 3' incomplete, TSL:3, |
| SMARCB1-203 | ENST00000407082.4 | 595 | 147aa | Protein coding | - | B5MCL5 | - | CDS 3' incomplete, TSL:5, |
| SMARCB1-208 | ENST00000634926.1 | 269 | 90aa | Protein coding | - | A0A0U1RRB8 | - | CDS 5' and 3' incomplete, TSL:1, |
| SMARCB1-209 | ENST00000635578.1 | 242 | 81aa | Protein coding | - | A0A0U1RQQ2 | - | CDS 5' and 3' incomplete, TSL:1, |
| SMARCB1-214 | ENST00000644462.1 | 2054 | 216aa | Nonsense mediated decay | - | A0A2R8YEE8 | - | CDS 5' incomplete, |
| SMARCB1-216 | ENST00000644619.1 | 1838 | 271aa | Nonsense mediated decay | - | A0A2R8Y5N5 | - | - |
| SMARCB1-221 | ENST00000647057.1 | 1401 | 78aa | Nonsense mediated decay | - | A0A2R8YDN6 | - | - |
| SMARCB1-211 | ENST00000642727.1 | 1001 | 32aa | Nonsense mediated decay | - | A0A2R8Y5Z2 | - | CDS 5' incomplete, |
| SMARCB1-219 | ENST00000646723.1 | 3594 | No protein | Retained intron | - | - | - | - |
| SMARCB1-218 | ENST00000646421.1 | 3550 | No protein | Retained intron | - | - | - | - |
| SMARCB1-215 | ENST00000644467.1 | 3322 | No protein | Retained intron | - | - | - | - |
| SMARCB1-217 | ENST00000645799.1 | 2653 | No protein | Retained intron | - | - | - | - |
| SMARCB1-206 | ENST00000477836.2 | 2153 | No protein | Retained intron | - | - | - | TSL:2, |
| SMARCB1-220 | ENST00000646911.1 | 1731 | No protein | Retained intron | - | - | - | - |
| SMARCB1-207 | ENST00000491967.2 | 1642 | No protein | Retained intron | - | - | - | TSL:1, |
| SMARCB1-212 | ENST00000643421.1 | 1557 | No protein | Retained intron | - | - | - | - |
| SMARCB1-210 | ENST00000642275.1 | 1285 | No protein | Retained intron | - | - | - | - |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SMARCB1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SMARCB1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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