CRISPR-U™ Gene Knockout Cell Line Strategy

SMARCB1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human SMARCB1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SMARCB1
Gene id 6598
Organism Homo sapiens
Official full symbol SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1
Gene type protein-coding
Also known as BAF47, CSS3, INI1, MRD15, PPP1R144, RDT, RTPS1, SNF5, SNF5L1, SWNTS1, Sfh1p, Snr1, hSNFS
Summary The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Alternatively spliced transcript variants have been found for this gene.
Genomic regions Chromosome 22
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
SMARCB1-213 ENST00000644036.2 5191 385aa Protein coding CCDS13817 Q12824-1 NM_003073.5 GENCODE basic, APPRIS ALT1, MANE Select v0.92,
SMARCB1-204 ENST00000407422.8 1730 376aa Protein coding CCDS46671 Q12824-2 - TSL:1, GENCODE basic, APPRIS P4,
SMARCB1-202 ENST00000344921.11 1717 394aa Protein coding CCDS82699 G5E975 - TSL:2, GENCODE basic,
SMARCB1-201 ENST00000263121.12 1500 339aa Protein coding - A0A0G2JRV3 - TSL:1, GENCODE basic,
SMARCB1-205 ENST00000417137.6 889 248aa Protein coding - C9JTA6 - CDS 3' incomplete, TSL:3,
SMARCB1-203 ENST00000407082.4 595 147aa Protein coding - B5MCL5 - CDS 3' incomplete, TSL:5,
SMARCB1-208 ENST00000634926.1 269 90aa Protein coding - A0A0U1RRB8 - CDS 5' and 3' incomplete, TSL:1,
SMARCB1-209 ENST00000635578.1 242 81aa Protein coding - A0A0U1RQQ2 - CDS 5' and 3' incomplete, TSL:1,
SMARCB1-214 ENST00000644462.1 2054 216aa Nonsense mediated decay - A0A2R8YEE8 - CDS 5' incomplete,
SMARCB1-216 ENST00000644619.1 1838 271aa Nonsense mediated decay - A0A2R8Y5N5 - -
SMARCB1-221 ENST00000647057.1 1401 78aa Nonsense mediated decay - A0A2R8YDN6 - -
SMARCB1-211 ENST00000642727.1 1001 32aa Nonsense mediated decay - A0A2R8Y5Z2 - CDS 5' incomplete,
SMARCB1-219 ENST00000646723.1 3594 No protein Retained intron - - - -
SMARCB1-218 ENST00000646421.1 3550 No protein Retained intron - - - -
SMARCB1-215 ENST00000644467.1 3322 No protein Retained intron - - - -
SMARCB1-217 ENST00000645799.1 2653 No protein Retained intron - - - -
SMARCB1-206 ENST00000477836.2 2153 No protein Retained intron - - - TSL:2,
SMARCB1-220 ENST00000646911.1 1731 No protein Retained intron - - - -
SMARCB1-207 ENST00000491967.2 1642 No protein Retained intron - - - TSL:1,
SMARCB1-212 ENST00000643421.1 1557 No protein Retained intron - - - -
SMARCB1-210 ENST00000642275.1 1285 No protein Retained intron - - - -
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SMARCB1
This KO Strategy loading
Red Cotton™ Notes Gene SMARCB1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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