CRISPR-U™ Gene Knockout Cell Line
Strategy
BMPR1A Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BMPR1A Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BMPR1A |
| Gene id | 657 |
| Organism | Homo sapiens |
| Official full symbol | bone morphogenetic protein receptor type 1A |
| Gene type | protein-coding |
| Also known as | 10q23del, ACVRLK3, ALK3, CD292, SKR5 |
| Summary | The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BMPR1A-201 | ENST00000372037.8 | 6417 | 532aa | Protein coding | CCDS7378 | P36894 | NM_004329.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BMPR1A-202 | ENST00000480152.2 | 421 | 41aa | Protein coding | - | A0A087X0P8 | - | CDS 3' incomplete, TSL:3, |
| BMPR1A-205 | ENST00000638429.1 | 5632 | 532aa | Nonsense mediated decay | CCDS7378 | P36894 | - | TSL:5, |
| BMPR1A-203 | ENST00000635816.1 | 3238 | 532aa | Nonsense mediated decay | CCDS7378 | P36894 | - | TSL:5, |
| BMPR1A-204 | ENST00000636056.1 | 3233 | 532aa | Nonsense mediated decay | CCDS7378 | P36894 | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BMPR1A |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BMPR1A had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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