CRISPR-U™ Gene Knockout Cell Line Strategy

LGALS9C Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LGALS9C Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LGALS9C
Gene id 654346
Organism Homo sapiens
Official full symbol galectin 9C
Gene type protein-coding
Also known as Gal-9B, LGALS9B
Summary This gene was initially thought to represent a pseudogene of galectin 9; however, this transcript has good exon-intron structure and encodes a predicted protein of the same size as and highly similar to galectin 9. This gene is one of two similar loci on chromosome 17p similar to galectin 9 and now thought to be protein-encoding. This gene is the more telomeric gene.
Genomic regions Chromosome 17
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LGALS9C-201 ENST00000328114.11 1715 356aa Protein coding CCDS32587 Q6DKI2 NM_001040078.3 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
LGALS9C-212 ENST00000583322.5 1602 323aa Protein coding - J3KSY2 - TSL:5, GENCODE basic, APPRIS ALT1,
LGALS9C-209 ENST00000581545.5 1378 246aa Protein coding - J3QR96 - TSL:5, GENCODE basic,
LGALS9C-215 ENST00000584941.5 1104 234aa Protein coding - J3KT17 - TSL:5, GENCODE basic,
LGALS9C-210 ENST00000582333.5 615 175aa Protein coding - J3QSH0 - CDS 3' incomplete, TSL:5,
LGALS9C-208 ENST00000580674.5 1515 75aa Nonsense mediated decay - J3KRF0 - TSL:5,
LGALS9C-203 ENST00000577691.5 1506 75aa Nonsense mediated decay - J3KRF0 - TSL:5,
LGALS9C-207 ENST00000579773.5 1117 35aa Nonsense mediated decay - J3QKS2 - TSL:2,
LGALS9C-205 ENST00000578563.1 381 53aa Nonsense mediated decay - J3KSV0 - CDS 5' incomplete, TSL:5,
LGALS9C-206 ENST00000578983.1 447 No protein Processed transcript - - - TSL:5,
LGALS9C-213 ENST00000584127.1 2947 No protein Retained intron - - - TSL:2,
LGALS9C-202 ENST00000577265.5 2354 No protein Retained intron - - - TSL:1,
LGALS9C-211 ENST00000582928.1 555 No protein Retained intron - - - TSL:5,
LGALS9C-214 ENST00000584170.5 549 No protein Retained intron - - - TSL:5,
LGALS9C-204 ENST00000578450.1 492 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LGALS9C
This KO Strategy loading
Red Cotton™ Notes Gene LGALS9C had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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