CRISPR-U™ Gene Knockout Cell Line
Strategy
LGALS9C Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LGALS9C Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LGALS9C |
| Gene id | 654346 |
| Organism | Homo sapiens |
| Official full symbol | galectin 9C |
| Gene type | protein-coding |
| Also known as | Gal-9B, LGALS9B |
| Summary | This gene was initially thought to represent a pseudogene of galectin 9; however, this transcript has good exon-intron structure and encodes a predicted protein of the same size as and highly similar to galectin 9. This gene is one of two similar loci on chromosome 17p similar to galectin 9 and now thought to be protein-encoding. This gene is the more telomeric gene. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LGALS9C-201 | ENST00000328114.11 | 1715 | 356aa | Protein coding | CCDS32587 | Q6DKI2 | NM_001040078.3 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| LGALS9C-212 | ENST00000583322.5 | 1602 | 323aa | Protein coding | - | J3KSY2 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| LGALS9C-209 | ENST00000581545.5 | 1378 | 246aa | Protein coding | - | J3QR96 | - | TSL:5, GENCODE basic, |
| LGALS9C-215 | ENST00000584941.5 | 1104 | 234aa | Protein coding | - | J3KT17 | - | TSL:5, GENCODE basic, |
| LGALS9C-210 | ENST00000582333.5 | 615 | 175aa | Protein coding | - | J3QSH0 | - | CDS 3' incomplete, TSL:5, |
| LGALS9C-208 | ENST00000580674.5 | 1515 | 75aa | Nonsense mediated decay | - | J3KRF0 | - | TSL:5, |
| LGALS9C-203 | ENST00000577691.5 | 1506 | 75aa | Nonsense mediated decay | - | J3KRF0 | - | TSL:5, |
| LGALS9C-207 | ENST00000579773.5 | 1117 | 35aa | Nonsense mediated decay | - | J3QKS2 | - | TSL:2, |
| LGALS9C-205 | ENST00000578563.1 | 381 | 53aa | Nonsense mediated decay | - | J3KSV0 | - | CDS 5' incomplete, TSL:5, |
| LGALS9C-206 | ENST00000578983.1 | 447 | No protein | Processed transcript | - | - | - | TSL:5, |
| LGALS9C-213 | ENST00000584127.1 | 2947 | No protein | Retained intron | - | - | - | TSL:2, |
| LGALS9C-202 | ENST00000577265.5 | 2354 | No protein | Retained intron | - | - | - | TSL:1, |
| LGALS9C-211 | ENST00000582928.1 | 555 | No protein | Retained intron | - | - | - | TSL:5, |
| LGALS9C-214 | ENST00000584170.5 | 549 | No protein | Retained intron | - | - | - | TSL:5, |
| LGALS9C-204 | ENST00000578450.1 | 492 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LGALS9C |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LGALS9C had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
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$599
Single gRNA lentivirus
Work flow
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