CRISPR-U™ Gene Knockout Cell Line
Strategy
SIPA1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SIPA1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SIPA1 |
| Gene id | 6494 |
| Organism | Homo sapiens |
| Official full symbol | signal-induced proliferation-associated 1 |
| Gene type | protein-coding |
| Also known as | SPA1 |
| Summary | The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SIPA1-201 | ENST00000394224.3 | 3628 | 1042aa | Protein coding | CCDS8108 | Q96FS4 | - | TSL:1, GENCODE basic, APPRIS P2, |
| SIPA1-209 | ENST00000534313.6 | 3499 | 1042aa | Protein coding | CCDS8108 | Q96FS4 | NM_006747.4 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| SIPA1-203 | ENST00000527525.5 | 3235 | 940aa | Protein coding | - | F6RY50 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| SIPA1-208 | ENST00000533361.1 | 562 | 102aa | Protein coding | - | E9PIB3 | - | CDS 3' incomplete, TSL:4, |
| SIPA1-202 | ENST00000526137.1 | 379 | 6aa | Protein coding | - | - | - | CDS 3' incomplete, TSL:3, |
| SIPA1-207 | ENST00000531339.5 | 799 | No protein | Retained intron | - | - | - | TSL:5, |
| SIPA1-206 | ENST00000530226.1 | 560 | No protein | Retained intron | - | - | - | TSL:2, |
| SIPA1-204 | ENST00000528699.1 | 548 | No protein | Retained intron | - | - | - | TSL:2, |
| SIPA1-205 | ENST00000529725.1 | 435 | No protein | Retained intron | - | - | - | TSL:2, |
| SIPA1-210 | ENST00000534406.1 | 351 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SIPA1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SIPA1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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