CRISPR-U™ Gene Knockout Cell Line Strategy

SIPA1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human SIPA1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SIPA1
Gene id 6494
Organism Homo sapiens
Official full symbol signal-induced proliferation-associated 1
Gene type protein-coding
Also known as SPA1
Summary The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date.
Genomic regions Chromosome 11
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
SIPA1-201 ENST00000394224.3 3628 1042aa Protein coding CCDS8108 Q96FS4 - TSL:1, GENCODE basic, APPRIS P2,
SIPA1-209 ENST00000534313.6 3499 1042aa Protein coding CCDS8108 Q96FS4 NM_006747.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
SIPA1-203 ENST00000527525.5 3235 940aa Protein coding - F6RY50 - TSL:2, GENCODE basic, APPRIS ALT2,
SIPA1-208 ENST00000533361.1 562 102aa Protein coding - E9PIB3 - CDS 3' incomplete, TSL:4,
SIPA1-202 ENST00000526137.1 379 6aa Protein coding - - - CDS 3' incomplete, TSL:3,
SIPA1-207 ENST00000531339.5 799 No protein Retained intron - - - TSL:5,
SIPA1-206 ENST00000530226.1 560 No protein Retained intron - - - TSL:2,
SIPA1-204 ENST00000528699.1 548 No protein Retained intron - - - TSL:2,
SIPA1-205 ENST00000529725.1 435 No protein Retained intron - - - TSL:2,
SIPA1-210 ENST00000534406.1 351 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SIPA1
This KO Strategy loading
Red Cotton™ Notes Gene SIPA1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×