CRISPR-U™ Gene Knockout Cell Line
Strategy
BMI1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BMI1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BMI1 |
| Gene id | 648 |
| Organism | Homo sapiens |
| Official full symbol | BMI1 proto-oncogene, polycomb ring finger |
| Gene type | protein-coding |
| Also known as | FLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1 |
| Summary | This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BMI1-201 | ENST00000376663.8 | 3540 | 326aa | Protein coding | CCDS7138 | P35226 | NM_005180.9 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BMI1-204 | ENST00000443519.1 | 552 | 184aa | Protein coding | - | Q5T8Z2 | - | CDS 5' and 3' incomplete, TSL:3, |
| BMI1-203 | ENST00000442508.5 | 536 | 150aa | Protein coding | - | Q5T8Z4 | - | CDS 3' incomplete, TSL:5, |
| BMI1-202 | ENST00000416820.5 | 406 | 105aa | Protein coding | - | Q5T8Z6 | - | CDS 3' incomplete, TSL:5, |
| BMI1-208 | ENST00000602358.5 | 943 | No protein | Retained intron | - | - | - | TSL:3, |
| BMI1-209 | ENST00000602523.1 | 748 | No protein | Retained intron | - | - | - | TSL:1, |
| BMI1-206 | ENST00000490311.1 | 693 | No protein | Retained intron | - | - | - | TSL:3, |
| BMI1-205 | ENST00000456675.2 | 550 | No protein | Retained intron | - | - | - | TSL:2, |
| BMI1-210 | ENST00000602524.1 | 545 | No protein | Retained intron | - | - | - | TSL:3, |
| BMI1-207 | ENST00000496174.5 | 365 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BMI1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BMI1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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