CRISPR-U™ Gene Knockout Cell Line Strategy

BMI1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human BMI1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BMI1
Gene id 648
Organism Homo sapiens
Official full symbol BMI1 proto-oncogene, polycomb ring finger
Gene type protein-coding
Also known as FLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1
Summary This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene.
Genomic regions Chromosome 10
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BMI1-201 ENST00000376663.8 3540 326aa Protein coding CCDS7138 P35226 NM_005180.9 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
BMI1-204 ENST00000443519.1 552 184aa Protein coding - Q5T8Z2 - CDS 5' and 3' incomplete, TSL:3,
BMI1-203 ENST00000442508.5 536 150aa Protein coding - Q5T8Z4 - CDS 3' incomplete, TSL:5,
BMI1-202 ENST00000416820.5 406 105aa Protein coding - Q5T8Z6 - CDS 3' incomplete, TSL:5,
BMI1-208 ENST00000602358.5 943 No protein Retained intron - - - TSL:3,
BMI1-209 ENST00000602523.1 748 No protein Retained intron - - - TSL:1,
BMI1-206 ENST00000490311.1 693 No protein Retained intron - - - TSL:3,
BMI1-205 ENST00000456675.2 550 No protein Retained intron - - - TSL:2,
BMI1-210 ENST00000602524.1 545 No protein Retained intron - - - TSL:3,
BMI1-207 ENST00000496174.5 365 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BMI1
This KO Strategy loading
Red Cotton™ Notes Gene BMI1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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