CRISPR-U™ Gene Knockout Cell Line
Strategy
IPPK Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IPPK Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IPPK |
| Gene id | 64768 |
| Organism | Homo sapiens |
| Official full symbol | inositol-pentakisphosphate 2-kinase |
| Gene type | protein-coding |
| Also known as | C9orf12, INSP5K2, IP5K, IPK1, bA476B13.1 |
| Summary | The protein encoded by this gene is a kinase that phosphorylates position 2 of inositol-1,3,4,5,6-pentakisphosphate to form inositol-1,2,3,4,5,6-hexakisphosphate (InsP6). InsP6 has a variety of functions, including stimulation of DNA repair, endocytosis, and mRNA export. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IPPK-201 | ENST00000287996.8 | 4268 | 491aa | Protein coding | CCDS6699 | Q9H8X2 | NM_022755.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| IPPK-202 | ENST00000375522.1 | 882 | 163aa | Protein coding | - | X6R9A6 | - | TSL:3, GENCODE basic, |
| IPPK-203 | ENST00000486841.1 | 2347 | No protein | Processed transcript | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IPPK |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IPPK had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: