CRISPR-U™ Gene Knockout Cell Line
Strategy
CREB3L2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CREB3L2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CREB3L2 |
| Gene id | 64764 |
| Organism | Homo sapiens |
| Official full symbol | cAMP responsive element binding protein 3 like 2 |
| Gene type | protein-coding |
| Also known as | BBF2H7 |
| Summary | This gene encodes a member of the oasis bZIP transcription factor family. Members of this family can dimerize but form homodimers only. The encoded protein is a transcriptional activator. Translocations between this gene on chromosome 7 and the gene fused in sarcoma on chromosome 16 can be found in some tumors. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CREB3L2-201 | ENST00000330387.11 | 7441 | 520aa | Protein coding | CCDS34760 | Q70SY1-1 | NM_194071.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CREB3L2-203 | ENST00000452463.5 | 1105 | 248aa | Protein coding | CCDS59083 | Q70SY1-3 | - | TSL:1, GENCODE basic, |
| CREB3L2-207 | ENST00000616381.4 | 7133 | 150aa | Protein coding | - | A0A087WU77 | - | TSL:5, GENCODE basic, |
| CREB3L2-204 | ENST00000456390.5 | 2360 | 460aa | Protein coding | - | Q70SY1-2 | - | TSL:2, GENCODE basic, |
| CREB3L2-208 | ENST00000620715.4 | 1053 | 175aa | Protein coding | - | A0A087WUP1 | - | TSL:5, GENCODE basic, |
| CREB3L2-205 | ENST00000458726.5 | 776 | 185aa | Protein coding | - | C9JPH0 | - | TSL:3, GENCODE basic, |
| CREB3L2-202 | ENST00000420629.1 | 719 | 239aa | Protein coding | - | H7C1W8 | - | CDS 5' and 3' incomplete, TSL:3, |
| CREB3L2-206 | ENST00000468127.1 | 596 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CREB3L2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CREB3L2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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