CRISPR-U™ Gene Knockout Cell Line Strategy

SMURF2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human SMURF2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SMURF2
Gene id 64750
Organism Homo sapiens
Official full symbol SMAD specific E3 ubiquitin protein ligase 2
Gene type protein-coding
Genomic regions Chromosome 17
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
SMURF2-201 ENST00000262435.14 6240 748aa Protein coding CCDS32707 Q9HAU4 NM_022739.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
SMURF2-207 ENST00000585301.1 1716 561aa Protein coding - J3QQM4 - CDS 3' incomplete, TSL:5,
SMURF2-206 ENST00000582081.5 5892 55aa Nonsense mediated decay - J3QLG1 - TSL:5,
SMURF2-203 ENST00000578386.5 3104 68aa Nonsense mediated decay - J3QRY2 - TSL:1,
SMURF2-202 ENST00000578200.1 339 No protein Processed transcript - - - TSL:5,
SMURF2-205 ENST00000580246.1 623 No protein Retained intron - - - TSL:2,
SMURF2-204 ENST00000580072.5 581 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SMURF2
This KO Strategy loading
Red Cotton™ Notes Gene SMURF2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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