CRISPR-U™ Gene Knockout Cell Line
Strategy
SMURF2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SMURF2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SMURF2 |
| Gene id | 64750 |
| Organism | Homo sapiens |
| Official full symbol | SMAD specific E3 ubiquitin protein ligase 2 |
| Gene type | protein-coding |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SMURF2-201 | ENST00000262435.14 | 6240 | 748aa | Protein coding | CCDS32707 | Q9HAU4 | NM_022739.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| SMURF2-207 | ENST00000585301.1 | 1716 | 561aa | Protein coding | - | J3QQM4 | - | CDS 3' incomplete, TSL:5, |
| SMURF2-206 | ENST00000582081.5 | 5892 | 55aa | Nonsense mediated decay | - | J3QLG1 | - | TSL:5, |
| SMURF2-203 | ENST00000578386.5 | 3104 | 68aa | Nonsense mediated decay | - | J3QRY2 | - | TSL:1, |
| SMURF2-202 | ENST00000578200.1 | 339 | No protein | Processed transcript | - | - | - | TSL:5, |
| SMURF2-205 | ENST00000580246.1 | 623 | No protein | Retained intron | - | - | - | TSL:2, |
| SMURF2-204 | ENST00000580072.5 | 581 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SMURF2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SMURF2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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