CRISPR-U™ Gene Knockout Cell Line Strategy

INF2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human INF2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol INF2
Gene id 64423
Organism Homo sapiens
Official full symbol inverted formin 2
Gene type protein-coding
Also known as C14orf151, C14orf173, CMTDIE, FSGS5, pp9484
Summary This gene represents a member of the formin family of proteins. It is considered a diaphanous formin due to the presence of a diaphanous inhibitory domain located at the N-terminus of the encoded protein. Studies of a similar mouse protein indicate that the protein encoded by this locus may function in polymerization and depolymerization of actin filaments. Mutations at this locus have been associated with focal segmental glomerulosclerosis 5.
Genomic regions Chromosome 14
Strategy Summary
This gene has 15 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
INF2-203 ENST00000392634.9 7623 1249aa Protein coding CCDS9989 Q27J81-1 NM_022489.4 TSL:5, GENCODE basic, APPRIS P4, MANE Select v0.92,
INF2-202 ENST00000330634.11 7578 1240aa Protein coding CCDS45173 Q27J81-2 - TSL:5, GENCODE basic, APPRIS ALT2,
INF2-204 ENST00000398337.8 1689 234aa Protein coding CCDS41999 Q27J81-3 - TSL:1, GENCODE basic,
INF2-227 ENST00000675207.1 7732 1281aa Protein coding - - - GENCODE basic, APPRIS ALT2,
INF2-238 ENST00000675638.1 7411 1222aa Protein coding - - - GENCODE basic, APPRIS ALT2,
INF2-245 ENST00000675980.1 3768 1255aa Protein coding - - - GENCODE basic, APPRIS ALT2,
INF2-232 ENST00000675481.1 3754 1250aa Protein coding - - - GENCODE basic, APPRIS ALT2,
INF2-230 ENST00000675329.1 3726 1241aa Protein coding - - - GENCODE basic,
INF2-224 ENST00000674991.1 2944 981aa Protein coding - - - CDS 3' incomplete,
INF2-213 ENST00000674631.1 2200 614aa Protein coding - - - CDS 5' incomplete,
INF2-233 ENST00000675482.1 1020 130aa Protein coding - - - CDS 3' incomplete,
INF2-216 ENST00000674723.1 931 130aa Protein coding - - - CDS 3' incomplete,
INF2-250 ENST00000676427.1 883 130aa Protein coding - - - CDS 3' incomplete,
INF2-221 ENST00000674869.1 837 130aa Protein coding - - - CDS 3' incomplete,
INF2-212 ENST00000674602.1 806 269aa Protein coding - - - CDS 5' and 3' incomplete,
INF2-210 ENST00000617571.5 4556 1048aa Nonsense mediated decay - Q8WYS3 - TSL:1,
INF2-217 ENST00000674757.1 3756 1051aa Nonsense mediated decay - - - -
INF2-214 ENST00000674662.1 3755 776aa Nonsense mediated decay - - - -
INF2-243 ENST00000675809.1 3749 793aa Nonsense mediated decay - - - -
INF2-211 ENST00000674520.1 3689 878aa Nonsense mediated decay - - - -
INF2-219 ENST00000674846.1 3689 773aa Nonsense mediated decay - - - -
INF2-220 ENST00000674857.1 3683 194aa Nonsense mediated decay - - - -
INF2-244 ENST00000675930.1 3639 1160aa Nonsense mediated decay - - - -
INF2-239 ENST00000675724.1 3632 646aa Nonsense mediated decay - - - -
INF2-235 ENST00000675583.1 3623 751aa Nonsense mediated decay - - - -
INF2-246 ENST00000676016.1 3593 741aa Nonsense mediated decay - - - -
INF2-218 ENST00000674822.1 3578 159aa Nonsense mediated decay - - - -
INF2-249 ENST00000676366.1 3558 1133aa Nonsense mediated decay - - - -
INF2-222 ENST00000674960.1 3552 316aa Nonsense mediated decay - - - -
INF2-242 ENST00000675797.1 3099 358aa Nonsense mediated decay - - - -
INF2-201 ENST00000252527.8 3050 717aa Nonsense mediated decay - A0A0A0MQU1 - CDS 5' incomplete, TSL:2,
INF2-225 ENST00000674994.1 3006 230aa Nonsense mediated decay - - - -
INF2-241 ENST00000675771.1 2957 332aa Nonsense mediated decay - - - -
INF2-231 ENST00000675424.1 1051 18aa Nonsense mediated decay - - - CDS 5' incomplete,
INF2-215 ENST00000674686.1 967 18aa Nonsense mediated decay - - - CDS 5' incomplete,
INF2-209 ENST00000540171.2 1307 No protein Processed transcript - - - TSL:1,
INF2-208 ENST00000481338.1 1102 No protein Processed transcript - - - TSL:2,
INF2-236 ENST00000675603.1 934 No protein Processed transcript - - - -
INF2-223 ENST00000674966.1 859 No protein Processed transcript - - - -
INF2-240 ENST00000675743.1 648 No protein Processed transcript - - - -
INF2-228 ENST00000675248.1 577 No protein Processed transcript - - - -
INF2-247 ENST00000676100.1 528 No protein Processed transcript - - - -
INF2-229 ENST00000675313.1 432 No protein Processed transcript - - - -
INF2-237 ENST00000675616.1 431 No protein Processed transcript - - - -
INF2-248 ENST00000676134.1 382 No protein Processed transcript - - - -
INF2-226 ENST00000675029.1 1692 No protein Retained intron - - - -
INF2-205 ENST00000474229.1 947 No protein Retained intron - - - TSL:3,
INF2-207 ENST00000480763.2 581 No protein Retained intron - - - TSL:3,
INF2-234 ENST00000675557.1 547 No protein Retained intron - - - -
INF2-206 ENST00000477497.1 545 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene INF2
This KO Strategy loading
Red Cotton™ Notes Gene INF2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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