CRISPR-U™ Gene Knockout Cell Line
Strategy
ARAP3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ARAP3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ARAP3 |
| Gene id | 64411 |
| Organism | Homo sapiens |
| Official full symbol | ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 |
| Gene type | protein-coding |
| Also known as | CENTD3, DRAG1 |
| Summary | This gene encodes a phosphoinositide binding protein containing ARF-GAP, RHO-GAP, RAS-associating, and pleckstrin homology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton and cell shape. It is a specific PtdIns(3,4,5)P3/PtdIns(3,4)P2-stimulated Arf6-GAP protein. An alternatively spliced transcript has been found for this gene, but its biological validity has not been determined. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ARAP3-201 | ENST00000239440.9 | 5258 | 1544aa | Protein coding | CCDS4266 | Q8WWN8-1 | NM_022481.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ARAP3-207 | ENST00000626478.2 | 4928 | 1375aa | Protein coding | - | G5E9Y3 | - | TSL:5, GENCODE basic, |
| ARAP3-203 | ENST00000508305.5 | 4927 | 1375aa | Protein coding | - | G5E9Y3 | - | TSL:2, GENCODE basic, |
| ARAP3-205 | ENST00000513878.5 | 3810 | 1193aa | Protein coding | - | Q8WWN8-2 | - | TSL:2, GENCODE basic, |
| ARAP3-202 | ENST00000504448.1 | 1765 | 473aa | Protein coding | - | Q05CH1 | - | CDS 3' incomplete, TSL:1, |
| ARAP3-204 | ENST00000512390.1 | 3179 | No protein | Processed transcript | - | - | - | TSL:2, |
| ARAP3-206 | ENST00000524066.1 | 533 | No protein | Processed transcript | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ARAP3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ARAP3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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