CRISPR-U™ Gene Knockout Cell Line Strategy

ARAP3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ARAP3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ARAP3
Gene id 64411
Organism Homo sapiens
Official full symbol ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3
Gene type protein-coding
Also known as CENTD3, DRAG1
Summary This gene encodes a phosphoinositide binding protein containing ARF-GAP, RHO-GAP, RAS-associating, and pleckstrin homology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton and cell shape. It is a specific PtdIns(3,4,5)P3/PtdIns(3,4)P2-stimulated Arf6-GAP protein. An alternatively spliced transcript has been found for this gene, but its biological validity has not been determined.
Genomic regions Chromosome 5
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ARAP3-201 ENST00000239440.9 5258 1544aa Protein coding CCDS4266 Q8WWN8-1 NM_022481.6 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ARAP3-207 ENST00000626478.2 4928 1375aa Protein coding - G5E9Y3 - TSL:5, GENCODE basic,
ARAP3-203 ENST00000508305.5 4927 1375aa Protein coding - G5E9Y3 - TSL:2, GENCODE basic,
ARAP3-205 ENST00000513878.5 3810 1193aa Protein coding - Q8WWN8-2 - TSL:2, GENCODE basic,
ARAP3-202 ENST00000504448.1 1765 473aa Protein coding - Q05CH1 - CDS 3' incomplete, TSL:1,
ARAP3-204 ENST00000512390.1 3179 No protein Processed transcript - - - TSL:2,
ARAP3-206 ENST00000524066.1 533 No protein Processed transcript - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ARAP3
This KO Strategy loading
Red Cotton™ Notes Gene ARAP3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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