CRISPR-U™ Gene Knockout Cell Line
Strategy
BLVRA Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BLVRA Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BLVRA |
| Gene id | 644 |
| Organism | Homo sapiens |
| Official full symbol | biliverdin reductase A |
| Gene type | protein-coding |
| Also known as | BLVR, BVR, BVRA |
| Summary | The protein encoded by this gene belongs to the biliverdin reductase family, members of which catalyze the conversion of biliverdin to bilirubin in the presence of NADPH or NADH. Mutations in this gene are associated with hyperbiliverdinemia. Alternatively spliced transcript variants have been found for this gene. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BLVRA-202 | ENST00000402924.5 | 1161 | 296aa | Protein coding | CCDS5472 | A0A140VJF4 P53004 | - | TSL:2, GENCODE basic, APPRIS P1, |
| BLVRA-201 | ENST00000265523.9 | 1074 | 296aa | Protein coding | CCDS5472 | A0A140VJF4 P53004 | NM_000712.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BLVRA-203 | ENST00000424330.1 | 443 | 102aa | Protein coding | - | C9J1E1 | - | CDS 3' incomplete, TSL:3, |
| BLVRA-204 | ENST00000453612.1 | 757 | No protein | Retained intron | - | - | - | TSL:2, |
| BLVRA-205 | ENST00000486984.1 | 324 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BLVRA |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BLVRA had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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