CRISPR-U™ Gene Knockout Cell Line
Strategy
SRSF6 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SRSF6 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SRSF6 |
| Gene id | 6431 |
| Organism | Homo sapiens |
| Official full symbol | serine and arginine rich splicing factor 6 |
| Gene type | protein-coding |
| Also known as | B52, HEL-S-91, SFRS6, SRP55 |
| Summary | The protein encoded by this gene is involved in mRNA splicing and may play a role in the determination of alternative splicing. The encoded nuclear protein belongs to the splicing factor SR family and has been shown to bind with and modulate another member of the family, SFRS12. Alternative splicing results in multiple transcript variants. In addition, two pseudogenes, one on chromosome 17 and the other on the X chromosome, have been found for this gene. |
| Genomic regions | Chromosome 20 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SRSF6-201 | ENST00000244020.5 | 4353 | 344aa | Protein coding | CCDS13318 | Q13247-1 | NM_006275.6 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| SRSF6-204 | ENST00000668808.1 | 1227 | 276aa | Protein coding | - | A0A590UJP7 | - | GENCODE basic, APPRIS ALT2, |
| SRSF6-205 | ENST00000670741.1 | 1077 | 226aa | Protein coding | - | A0A590UJK4 | - | GENCODE basic, APPRIS ALT2, |
| SRSF6-203 | ENST00000662078.1 | 1007 | 273aa | Protein coding | - | A0A590UK01 | - | GENCODE basic, APPRIS ALT2, |
| SRSF6-202 | ENST00000483871.6 | 1680 | 135aa | Nonsense mediated decay | - | Q13247-2 | - | TSL:2, |
| SRSF6-206 | ENST00000671022.1 | 1275 | No protein | Processed transcript | - | - | - | - |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SRSF6 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SRSF6 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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