CRISPR-U™ Gene Knockout Cell Line Strategy

SRSF6 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human SRSF6 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SRSF6
Gene id 6431
Organism Homo sapiens
Official full symbol serine and arginine rich splicing factor 6
Gene type protein-coding
Also known as B52, HEL-S-91, SFRS6, SRP55
Summary The protein encoded by this gene is involved in mRNA splicing and may play a role in the determination of alternative splicing. The encoded nuclear protein belongs to the splicing factor SR family and has been shown to bind with and modulate another member of the family, SFRS12. Alternative splicing results in multiple transcript variants. In addition, two pseudogenes, one on chromosome 17 and the other on the X chromosome, have been found for this gene.
Genomic regions Chromosome 20
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
SRSF6-201 ENST00000244020.5 4353 344aa Protein coding CCDS13318 Q13247-1 NM_006275.6 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
SRSF6-204 ENST00000668808.1 1227 276aa Protein coding - A0A590UJP7 - GENCODE basic, APPRIS ALT2,
SRSF6-205 ENST00000670741.1 1077 226aa Protein coding - A0A590UJK4 - GENCODE basic, APPRIS ALT2,
SRSF6-203 ENST00000662078.1 1007 273aa Protein coding - A0A590UK01 - GENCODE basic, APPRIS ALT2,
SRSF6-202 ENST00000483871.6 1680 135aa Nonsense mediated decay - Q13247-2 - TSL:2,
SRSF6-206 ENST00000671022.1 1275 No protein Processed transcript - - - -
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SRSF6
This KO Strategy loading
Red Cotton™ Notes Gene SRSF6 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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