CRISPR-U™ Gene Knockout Cell Line
Strategy
BLMH Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BLMH Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BLMH |
| Gene id | 642 |
| Organism | Homo sapiens |
| Official full symbol | bleomycin hydrolase |
| Gene type | protein-coding |
| Also known as | BH, BMH |
| Summary | Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BLMH-201 | ENST00000261714.11 | 2307 | 455aa | Protein coding | CCDS32604 | Q13867 | NM_000386.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BLMH-213 | ENST00000584603.2 | 1020 | 318aa | Protein coding | - | K7ES02 | - | CDS 3' incomplete, TSL:5, |
| BLMH-204 | ENST00000577623.5 | 823 | 231aa | Protein coding | - | K7ESE8 | - | CDS 3' incomplete, TSL:5, |
| BLMH-210 | ENST00000581037.5 | 788 | 262aa | Protein coding | - | J3KSD8 | - | CDS 5' and 3' incomplete, TSL:5, |
| BLMH-209 | ENST00000580709.1 | 561 | 67aa | Protein coding | - | K7EMJ3 | - | CDS 3' incomplete, TSL:4, |
| BLMH-205 | ENST00000578090.5 | 1483 | 71aa | Nonsense mediated decay | - | J3KS79 | - | TSL:2, |
| BLMH-202 | ENST00000577290.1 | 586 | 93aa | Nonsense mediated decay | - | K7ENH5 | - | TSL:4, |
| BLMH-211 | ENST00000582669.1 | 477 | No protein | Processed transcript | - | - | - | TSL:4, |
| BLMH-206 | ENST00000578795.1 | 2079 | No protein | Retained intron | - | - | - | TSL:NA, |
| BLMH-212 | ENST00000582749.1 | 769 | No protein | Retained intron | - | - | - | TSL:2, |
| BLMH-203 | ENST00000577306.1 | 761 | No protein | Retained intron | - | - | - | TSL:2, |
| BLMH-207 | ENST00000579325.1 | 585 | No protein | Retained intron | - | - | - | TSL:3, |
| BLMH-208 | ENST00000579957.1 | 293 | No protein | Retained intron | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BLMH |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BLMH had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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