CRISPR-U™ Gene Knockout Cell Line Strategy

BLMH Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BLMH Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BLMH
Gene id 642
Organism Homo sapiens
Official full symbol bleomycin hydrolase
Gene type protein-coding
Also known as BH, BMH
Summary Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily.
Genomic regions Chromosome 17
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BLMH-201 ENST00000261714.11 2307 455aa Protein coding CCDS32604 Q13867 NM_000386.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
BLMH-213 ENST00000584603.2 1020 318aa Protein coding - K7ES02 - CDS 3' incomplete, TSL:5,
BLMH-204 ENST00000577623.5 823 231aa Protein coding - K7ESE8 - CDS 3' incomplete, TSL:5,
BLMH-210 ENST00000581037.5 788 262aa Protein coding - J3KSD8 - CDS 5' and 3' incomplete, TSL:5,
BLMH-209 ENST00000580709.1 561 67aa Protein coding - K7EMJ3 - CDS 3' incomplete, TSL:4,
BLMH-205 ENST00000578090.5 1483 71aa Nonsense mediated decay - J3KS79 - TSL:2,
BLMH-202 ENST00000577290.1 586 93aa Nonsense mediated decay - K7ENH5 - TSL:4,
BLMH-211 ENST00000582669.1 477 No protein Processed transcript - - - TSL:4,
BLMH-206 ENST00000578795.1 2079 No protein Retained intron - - - TSL:NA,
BLMH-212 ENST00000582749.1 769 No protein Retained intron - - - TSL:2,
BLMH-203 ENST00000577306.1 761 No protein Retained intron - - - TSL:2,
BLMH-207 ENST00000579325.1 585 No protein Retained intron - - - TSL:3,
BLMH-208 ENST00000579957.1 293 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BLMH
This KO Strategy loading
Red Cotton™ Notes Gene BLMH had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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