CRISPR-U™ Gene Knockout Cell Line Strategy

MAP2K4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MAP2K4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MAP2K4
Gene id 6416
Organism Homo sapiens
Official full symbol mitogen-activated protein kinase kinase 4
Gene type protein-coding
Summary This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 17
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MAP2K4-202 ENST00000415385.7 3856 410aa Protein coding CCDS62095 P45985-2 - TSL:2, GENCODE basic, APPRIS ALT2,
MAP2K4-201 ENST00000353533.10 3778 399aa Protein coding CCDS11162 P45985-1 NM_003010.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
MAP2K4-215 ENST00000602811.5 1136 41aa Nonsense mediated decay - R4GN68 - TSL:5,
MAP2K4-212 ENST00000602375.5 1064 92aa Nonsense mediated decay - R4GN37 - TSL:5,
MAP2K4-214 ENST00000602686.5 977 41aa Nonsense mediated decay - R4GN68 - TSL:5,
MAP2K4-211 ENST00000602305.5 961 45aa Nonsense mediated decay - J3QLE2 - TSL:5,
MAP2K4-213 ENST00000602537.5 948 41aa Nonsense mediated decay - R4GN68 - TSL:5,
MAP2K4-204 ENST00000538465.7 925 16aa Nonsense mediated decay - K7EP58 - CDS 5' incomplete, TSL:5,
MAP2K4-207 ENST00000582183.5 551 45aa Nonsense mediated decay - J3QLE2 - TSL:4,
MAP2K4-206 ENST00000581941.5 813 No protein Processed transcript - - - TSL:3,
MAP2K4-205 ENST00000579089.5 571 No protein Processed transcript - - - TSL:4,
MAP2K4-209 ENST00000582897.5 482 No protein Processed transcript - - - TSL:5,
MAP2K4-203 ENST00000536413.2 3852 No protein Retained intron - - - TSL:2,
MAP2K4-208 ENST00000582377.1 648 No protein Retained intron - - - TSL:3,
MAP2K4-210 ENST00000585076.1 543 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MAP2K4
This KO Strategy loading
Red Cotton™ Notes Gene MAP2K4 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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