CRISPR-U™ Gene Knockout Cell Line
Strategy
MAP2K4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MAP2K4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MAP2K4 |
| Gene id | 6416 |
| Organism | Homo sapiens |
| Official full symbol | mitogen-activated protein kinase kinase 4 |
| Gene type | protein-coding |
| Also known as | JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1 |
| Summary | This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MAP2K4-202 | ENST00000415385.7 | 3856 | 410aa | Protein coding | CCDS62095 | P45985-2 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| MAP2K4-201 | ENST00000353533.10 | 3778 | 399aa | Protein coding | CCDS11162 | P45985-1 | NM_003010.4 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| MAP2K4-215 | ENST00000602811.5 | 1136 | 41aa | Nonsense mediated decay | - | R4GN68 | - | TSL:5, |
| MAP2K4-212 | ENST00000602375.5 | 1064 | 92aa | Nonsense mediated decay | - | R4GN37 | - | TSL:5, |
| MAP2K4-214 | ENST00000602686.5 | 977 | 41aa | Nonsense mediated decay | - | R4GN68 | - | TSL:5, |
| MAP2K4-211 | ENST00000602305.5 | 961 | 45aa | Nonsense mediated decay | - | J3QLE2 | - | TSL:5, |
| MAP2K4-213 | ENST00000602537.5 | 948 | 41aa | Nonsense mediated decay | - | R4GN68 | - | TSL:5, |
| MAP2K4-204 | ENST00000538465.7 | 925 | 16aa | Nonsense mediated decay | - | K7EP58 | - | CDS 5' incomplete, TSL:5, |
| MAP2K4-207 | ENST00000582183.5 | 551 | 45aa | Nonsense mediated decay | - | J3QLE2 | - | TSL:4, |
| MAP2K4-206 | ENST00000581941.5 | 813 | No protein | Processed transcript | - | - | - | TSL:3, |
| MAP2K4-205 | ENST00000579089.5 | 571 | No protein | Processed transcript | - | - | - | TSL:4, |
| MAP2K4-209 | ENST00000582897.5 | 482 | No protein | Processed transcript | - | - | - | TSL:5, |
| MAP2K4-203 | ENST00000536413.2 | 3852 | No protein | Retained intron | - | - | - | TSL:2, |
| MAP2K4-208 | ENST00000582377.1 | 648 | No protein | Retained intron | - | - | - | TSL:3, |
| MAP2K4-210 | ENST00000585076.1 | 543 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MAP2K4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MAP2K4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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