CRISPR-U™ Gene Knockout Cell Line Strategy

IFIH1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IFIH1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IFIH1
Gene id 64135
Organism Homo sapiens
Official full symbol interferon induced with helicase C domain 1
Gene type protein-coding
Also known as AGS7, Hlcd, IDDM19, MDA-5, MDA5, RLR-2, SGMRT1
Summary IFIH1 encodes MDA5 which is an intracellular sensor of viral RNA that triggers the innate immune response. Sensing RNA length and secondary structure, MDA5 binds dsRNA oligonucleotides with a modified DExD/H-box helicase core and a C-terminal domain, thus leading to a proinflammatory response that includes interferons. It has been shown that Coronaviruses (CoVs) as well as various other virus families, are capable of evading the MDA5-dependent interferon response, thus impeding the activation of the innate immune response to infection. MDA5 has also been shown to play an important role in enhancing natural killer cell function in malaria infection. In addition to its protective role in antiviral responses, MDA5 has been implicated in autoimmune and autoinflammatory diseases such as type 1 diabetes, systemic lupus erythematosus, and Aicardi-Goutieres syndrome
Genomic regions Chromosome 2
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IFIH1-206 ENST00000649979.2 3581 1025aa Protein coding CCDS2217 Q9BYX4-1 NM_022168.4 GENCODE basic, APPRIS P1, MANE Select v0.92,
IFIH1-203 ENST00000648433.1 3234 986aa Protein coding - A0A3B3IRK8 - GENCODE basic,
IFIH1-207 ENST00000679938.1 2964 921aa Protein coding - - - GENCODE basic,
IFIH1-201 ENST00000421365.2 1617 221aa Protein coding - Q9BYX4-2 - TSL:1, GENCODE basic,
IFIH1-205 ENST00000649554.1 2795 No protein Processed transcript - - - -
IFIH1-204 ENST00000649426.1 791 No protein Retained intron - - - -
IFIH1-202 ENST00000464129.1 567 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IFIH1
This KO Strategy loading
Red Cotton™ Notes Gene IFIH1 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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