CRISPR-U™ Gene Knockout Cell Line
Strategy
C19orf33 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human C19orf33 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | C19orf33 |
| Gene id | 64073 |
| Organism | Homo sapiens |
| Official full symbol | chromosome 19 open reading frame 33 |
| Gene type | protein-coding |
| Also known as | H2RSP, IMUP, IMUP-1, IMUP-2 |
| Summary | The protein encoded by this gene has been shown to be upregulated in SV40-immortalized fibroblasts as well as in endometrial carcinoma cells. The encoded protein is found primarily in the nucleus. This protein may play a role in placental development and diseases such as pre-eclampsia. Two transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| C19orf33-202 | ENST00000588605.5 | 520 | 85aa | Protein coding | CCDS82344 | Q9GZP8-2 | - | TSL:1, GENCODE basic, |
| C19orf33-201 | ENST00000301246.10 | 374 | 106aa | Protein coding | CCDS12511 | Q9GZP8-1 | NM_033520.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| C19orf33-204 | ENST00000591852.1 | 502 | No protein | Retained intron | - | - | - | TSL:2, |
| C19orf33-203 | ENST00000589986.2 | 462 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | C19orf33 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene C19orf33 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: