CRISPR-U™ Gene Knockout Cell Line Strategy

CCL20 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CCL20 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CCL20
Gene id 6364
Organism Homo sapiens
Official full symbol C-C motif chemokine ligand 20
Gene type protein-coding
Also known as CKb4, Exodus, LARC, MIP-3-alpha, MIP-3a, MIP3A, SCYA20, ST38
Summary This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 2
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CCL20-202 ENST00000409189.7 839 95aa Protein coding CCDS46536 P78556-2 - TSL:1, GENCODE basic, APPRIS P4,
CCL20-201 ENST00000358813.5 834 96aa Protein coding CCDS2469 P78556-1 NM_004591.3 TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92,
CCL20-205 ENST00000646475.1 397 25aa Protein coding - A0A2R8Y806 - CDS 3' incomplete,
CCL20-203 ENST00000473642.1 680 No protein Processed transcript - - - TSL:2,
CCL20-204 ENST00000489160.1 673 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CCL20
This KO Strategy loading
Red Cotton™ Notes Gene CCL20 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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