CRISPR-U™ Gene Knockout Cell Line
Strategy
CCL5 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CCL5 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CCL5 |
| Gene id | 6352 |
| Organism | Homo sapiens |
| Official full symbol | C-C motif chemokine ligand 5 |
| Gene type | protein-coding |
| Also known as | D17S136E, RANTES, SCYA5, SIS-delta, SISd, TCP228, eoCP |
| Summary | This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CCL5-201 | ENST00000603197.6 | 1352 | 91aa | Protein coding | CCDS11300 | D0EI67 P13501 | - | TSL:1, GENCODE basic, APPRIS P1, |
| CCL5-202 | ENST00000605140.6 | 1217 | 91aa | Protein coding | CCDS11300 | D0EI67 P13501 | - | TSL:5, GENCODE basic, APPRIS P1, |
| CCL5-203 | ENST00000605509.2 | 659 | 91aa | Protein coding | CCDS11300 | D0EI67 P13501 | - | TSL:3, GENCODE basic, APPRIS P1, |
| CCL5-204 | ENST00000651122.1 | 1299 | 154aa | Protein coding | - | A0A494C1Q1 | NM_001278736.2 | GENCODE basic, MANE Select v0.92, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CCL5 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CCL5 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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