CRISPR-U™ Gene Knockout Cell Line Strategy

CCL5 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CCL5 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CCL5
Gene id 6352
Organism Homo sapiens
Official full symbol C-C motif chemokine ligand 5
Gene type protein-coding
Also known as D17S136E, RANTES, SCYA5, SIS-delta, SISd, TCP228, eoCP
Summary This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms.
Genomic regions Chromosome 17
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CCL5-201 ENST00000603197.6 1352 91aa Protein coding CCDS11300 D0EI67 P13501 - TSL:1, GENCODE basic, APPRIS P1,
CCL5-202 ENST00000605140.6 1217 91aa Protein coding CCDS11300 D0EI67 P13501 - TSL:5, GENCODE basic, APPRIS P1,
CCL5-203 ENST00000605509.2 659 91aa Protein coding CCDS11300 D0EI67 P13501 - TSL:3, GENCODE basic, APPRIS P1,
CCL5-204 ENST00000651122.1 1299 154aa Protein coding - A0A494C1Q1 NM_001278736.2 GENCODE basic, MANE Select v0.92,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CCL5
This KO Strategy loading
Red Cotton™ Notes Gene CCL5 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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