CRISPR-U™ Gene Knockout Cell Line
Strategy
CCL2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CCL2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CCL2 |
| Gene id | 6347 |
| Organism | Homo sapiens |
| Official full symbol | C-C motif chemokine ligand 2 |
| Gene type | protein-coding |
| Also known as | GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, SCYA2, SMC-CF |
| Summary | This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CCL2-201 | ENST00000225831.4 | 741 | 99aa | Protein coding | CCDS11277 | P13500 | NM_002982.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CCL2-202 | ENST00000580907.5 | 736 | 65aa | Protein coding | - | J3KRT7 | - | TSL:2, GENCODE basic, |
| CCL2-203 | ENST00000582017.1 | 996 | No protein | Retained intron | - | - | - | TSL:NA, |
| CCL2-204 | ENST00000624362.1 | 992 | No protein | TEC | - | - | - | TSL:NA, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CCL2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CCL2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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