CRISPR-U™ Gene Knockout Cell Line Strategy

MAPK12 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MAPK12 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MAPK12
Gene id 6300
Organism Homo sapiens
Official full symbol mitogen-activated protein kinase 12
Gene type protein-coding
Also known as ERK-6, ERK3, ERK6, MAPK 12, P38GAMMA, PRKM12, SAPK-3, SAPK3
Summary Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. The protein encoded by this gene functions as a signal transducer during differentiation of myoblasts to myotubes.
Genomic regions Chromosome 22
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MAPK12-211 ENST00000622558.4 1779 357aa Protein coding CCDS77688 P53778-2 - TSL:1, GENCODE basic,
MAPK12-201 ENST00000215659.13 1778 367aa Protein coding CCDS14089 P53778-1 NM_002969.6 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MAPK12-203 ENST00000395780.5 1459 277aa Protein coding - B5MDL5 - TSL:1, GENCODE basic,
MAPK12-202 ENST00000395778.3 605 119aa Protein coding - A8MY48 - TSL:1, GENCODE basic,
MAPK12-209 ENST00000497036.5 6248 No protein Processed transcript - - - TSL:2,
MAPK12-207 ENST00000492218.1 498 No protein Processed transcript - - - TSL:4,
MAPK12-208 ENST00000496942.5 2763 No protein Retained intron - - - TSL:2,
MAPK12-205 ENST00000482969.5 2101 No protein Retained intron - - - TSL:2,
MAPK12-210 ENST00000497738.1 2021 No protein Retained intron - - - TSL:1,
MAPK12-204 ENST00000467891.5 2019 No protein Retained intron - - - TSL:5,
MAPK12-206 ENST00000488504.5 1785 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MAPK12
This KO Strategy loading
Red Cotton™ Notes Gene MAPK12 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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