CRISPR-U™ Gene Knockout Cell Line
Strategy
MAPK12 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MAPK12 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MAPK12 |
| Gene id | 6300 |
| Organism | Homo sapiens |
| Official full symbol | mitogen-activated protein kinase 12 |
| Gene type | protein-coding |
| Also known as | ERK-6, ERK3, ERK6, MAPK 12, P38GAMMA, PRKM12, SAPK-3, SAPK3 |
| Summary | Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. The protein encoded by this gene functions as a signal transducer during differentiation of myoblasts to myotubes. |
| Genomic regions | Chromosome 22 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MAPK12-211 | ENST00000622558.4 | 1779 | 357aa | Protein coding | CCDS77688 | P53778-2 | - | TSL:1, GENCODE basic, |
| MAPK12-201 | ENST00000215659.13 | 1778 | 367aa | Protein coding | CCDS14089 | P53778-1 | NM_002969.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MAPK12-203 | ENST00000395780.5 | 1459 | 277aa | Protein coding | - | B5MDL5 | - | TSL:1, GENCODE basic, |
| MAPK12-202 | ENST00000395778.3 | 605 | 119aa | Protein coding | - | A8MY48 | - | TSL:1, GENCODE basic, |
| MAPK12-209 | ENST00000497036.5 | 6248 | No protein | Processed transcript | - | - | - | TSL:2, |
| MAPK12-207 | ENST00000492218.1 | 498 | No protein | Processed transcript | - | - | - | TSL:4, |
| MAPK12-208 | ENST00000496942.5 | 2763 | No protein | Retained intron | - | - | - | TSL:2, |
| MAPK12-205 | ENST00000482969.5 | 2101 | No protein | Retained intron | - | - | - | TSL:2, |
| MAPK12-210 | ENST00000497738.1 | 2021 | No protein | Retained intron | - | - | - | TSL:1, |
| MAPK12-204 | ENST00000467891.5 | 2019 | No protein | Retained intron | - | - | - | TSL:5, |
| MAPK12-206 | ENST00000488504.5 | 1785 | No protein | Retained intron | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MAPK12 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MAPK12 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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