CRISPR-U™ Gene Knockout Cell Line Strategy

S100A4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human S100A4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol S100A4
Gene id 6275
Organism Homo sapiens
Official full symbol S100 calcium binding protein A4
Gene type protein-coding
Also known as 18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98
Summary The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Genomic regions Chromosome 1
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
S100A4-203 ENST00000368715.5 573 101aa Protein coding CCDS1042 P26447 - TSL:2, GENCODE basic, APPRIS P1,
S100A4-201 ENST00000354332.8 566 101aa Protein coding CCDS1042 P26447 - TSL:1, GENCODE basic, APPRIS P1,
S100A4-202 ENST00000368714.1 531 101aa Protein coding CCDS1042 P26447 - TSL:3, GENCODE basic, APPRIS P1,
S100A4-204 ENST00000368716.9 513 101aa Protein coding CCDS1042 P26447 NM_002961.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
S100A4-206 ENST00000481009.1 755 No protein Processed transcript - - - TSL:1,
S100A4-205 ENST00000468373.1 537 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene S100A4
This KO Strategy loading
Red Cotton™ Notes Gene S100A4 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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