CRISPR-U™ Gene Knockout Cell Line
Strategy
SORT1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SORT1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SORT1 |
| Gene id | 6272 |
| Organism | Homo sapiens |
| Official full symbol | sortilin 1 |
| Gene type | protein-coding |
| Also known as | Gp95, LDLCQ6, NT3, NTR3 |
| Summary | This gene encodes a member of the VPS10-related sortilin family of proteins. The encoded preproprotein is proteolytically processed by furin to generate the mature receptor. This receptor plays a role in the trafficking of different proteins to either the cell surface, or subcellular compartments such as lysosomes and endosomes. Expression levels of this gene may influence the risk of myocardial infarction in human patients. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SORT1-209 | ENST00000538502.5 | 6993 | 694aa | Protein coding | CCDS55618 | Q99523-2 | - | TSL:2, GENCODE basic, |
| SORT1-201 | ENST00000256637.8 | 6990 | 831aa | Protein coding | CCDS798 | Q99523-1 | NM_002959.7 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| SORT1-204 | ENST00000482236.5 | 615 | 60aa | Protein coding | - | A0A0J9YVX1 | - | CDS 3' incomplete, TSL:3, |
| SORT1-207 | ENST00000493736.5 | 583 | 118aa | Protein coding | - | A0A0J9YX61 | - | CDS 3' incomplete, TSL:2, |
| SORT1-210 | ENST00000633956.1 | 561 | 20aa | Protein coding | - | A0A0J9YY30 | - | CDS 3' incomplete, TSL:4, |
| SORT1-203 | ENST00000471996.1 | 469 | 104aa | Nonsense mediated decay | - | A0A0J9YVU2 | - | CDS 5' incomplete, TSL:3, |
| SORT1-206 | ENST00000485149.1 | 442 | No protein | Processed transcript | - | - | - | TSL:3, |
| SORT1-208 | ENST00000495777.5 | 391 | No protein | Processed transcript | - | - | - | TSL:2, |
| SORT1-205 | ENST00000483508.5 | 759 | No protein | Retained intron | - | - | - | TSL:3, |
| SORT1-202 | ENST00000466471.1 | 521 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SORT1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SORT1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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