CRISPR-U™ Gene Knockout Cell Line Strategy

SORT1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human SORT1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SORT1
Gene id 6272
Organism Homo sapiens
Official full symbol sortilin 1
Gene type protein-coding
Also known as Gp95, LDLCQ6, NT3, NTR3
Summary This gene encodes a member of the VPS10-related sortilin family of proteins. The encoded preproprotein is proteolytically processed by furin to generate the mature receptor. This receptor plays a role in the trafficking of different proteins to either the cell surface, or subcellular compartments such as lysosomes and endosomes. Expression levels of this gene may influence the risk of myocardial infarction in human patients. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 1
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
SORT1-209 ENST00000538502.5 6993 694aa Protein coding CCDS55618 Q99523-2 - TSL:2, GENCODE basic,
SORT1-201 ENST00000256637.8 6990 831aa Protein coding CCDS798 Q99523-1 NM_002959.7 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
SORT1-204 ENST00000482236.5 615 60aa Protein coding - A0A0J9YVX1 - CDS 3' incomplete, TSL:3,
SORT1-207 ENST00000493736.5 583 118aa Protein coding - A0A0J9YX61 - CDS 3' incomplete, TSL:2,
SORT1-210 ENST00000633956.1 561 20aa Protein coding - A0A0J9YY30 - CDS 3' incomplete, TSL:4,
SORT1-203 ENST00000471996.1 469 104aa Nonsense mediated decay - A0A0J9YVU2 - CDS 5' incomplete, TSL:3,
SORT1-206 ENST00000485149.1 442 No protein Processed transcript - - - TSL:3,
SORT1-208 ENST00000495777.5 391 No protein Processed transcript - - - TSL:2,
SORT1-205 ENST00000483508.5 759 No protein Retained intron - - - TSL:3,
SORT1-202 ENST00000466471.1 521 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SORT1
This KO Strategy loading
Red Cotton™ Notes Gene SORT1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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