CRISPR-U™ Gene Knockout Cell Line Strategy

BCR Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BCR Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BCR
Gene id 613
Organism Homo sapiens
Official full symbol BCR activator of RhoGEF and GTPase
Gene type protein-coding
Also known as ALL, BCR1, CML, D22S11, D22S662, PHL
Summary A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The unregulated tyrosine kinase activity of BCR-ABL1 contributes to the immortality of leukaemic cells. The BCR protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac and other kinases. Two transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 22
Strategy Summary
This gene has 3 protein coding transcripts：
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BCR-201 ENST00000305877.13 6783 1271aa Protein coding CCDS13806 P11274-1 NM_004327.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
BCR-202 ENST00000359540.7 4708 1227aa Protein coding CCDS13807 P11274-2 - TSL:1, GENCODE basic,
BCR-204 ENST00000427791.1 771 257aa Protein coding - H0Y554 - CDS 5' and 3' incomplete, TSL:1,
BCR-205 ENST00000436990.2 4188 No protein Processed transcript - - - TSL:2,
BCR-203 ENST00000419722.6 788 No protein Processed transcript - - - TSL:5,
BCR-206 ENST00000458056.2 778 No protein Processed transcript - - - TSL:5,
BCR-212 ENST00000479188.5 658 No protein Processed transcript - - - TSL:4,
BCR-209 ENST00000471452.1 638 No protein Processed transcript - - - TSL:3,
BCR-210 ENST00000475025.5 563 No protein Processed transcript - - - TSL:4,
BCR-213 ENST00000480973.1 535 No protein Processed transcript - - - TSL:4,
BCR-211 ENST00000478978.5 463 No protein Processed transcript - - - TSL:3,
BCR-214 ENST00000487679.1 231 No protein Processed transcript - - - TSL:1,
BCR-207 ENST00000463770.5 168 No protein Processed transcript - - - TSL:1,
BCR-215 ENST00000487968.5 2870 No protein Retained intron - - - TSL:2,
BCR-208 ENST00000466076.1 495 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BCR
This KO Strategy loading
Red Cotton™ Notes Gene BCR had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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