CRISPR-U™ Gene Knockout Cell Line
Strategy
FKBP10 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human FKBP10 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | FKBP10 |
| Gene id | 60681 |
| Organism | Homo sapiens |
| Official full symbol | FKBP prolyl isomerase 10 |
| Gene type | protein-coding |
| Also known as | BRKS1, FKBP65, OI11, OI6, PPIASE, hFKBP65 |
| Summary | The protein encoded by this gene belongs to the FKBP-type peptidyl-prolyl cis/trans isomerase (PPIase) family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone. Alternatively spliced variants encoding different isoforms have been reported, but their biological validity has not been determined. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| FKBP10-201 | ENST00000321562.9 | 2585 | 582aa | Protein coding | CCDS11409 | Q96AY3-1 | NM_021939.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| FKBP10-203 | ENST00000455106.1 | 1965 | 386aa | Protein coding | - | H0Y827 | - | CDS 5' incomplete, TSL:2, |
| FKBP10-208 | ENST00000585664.5 | 598 | 172aa | Protein coding | - | K7ESG6 | - | CDS 3' incomplete, TSL:3, |
| FKBP10-209 | ENST00000585922.5 | 563 | 171aa | Protein coding | - | K7ELI6 | - | CDS 3' incomplete, TSL:4, |
| FKBP10-202 | ENST00000429461.5 | 398 | 81aa | Protein coding | - | C9JPC3 | - | CDS 3' incomplete, TSL:3, |
| FKBP10-206 | ENST00000489591.5 | 2826 | 147aa | Nonsense mediated decay | - | K7EM43 | - | TSL:2, |
| FKBP10-204 | ENST00000464180.1 | 1129 | No protein | Retained intron | - | - | - | TSL:2, |
| FKBP10-207 | ENST00000490938.5 | 837 | No protein | Retained intron | - | - | - | TSL:2, |
| FKBP10-205 | ENST00000487489.1 | 578 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | FKBP10 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene FKBP10 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: