CRISPR-U™ Gene Knockout Cell Line Strategy

ELOVL5 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ELOVL5 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ELOVL5
Gene id 60481
Organism Homo sapiens
Official full symbol ELOVL fatty acid elongase 5
Gene type protein-coding
Also known as HELO1, SCA38, dJ483K16.1
Summary This gene belongs to the ELO family. It is highly expressed in the adrenal gland and testis, and encodes a multi-pass membrane protein that is localized in the endoplasmic reticulum. This protein is involved in the elongation of long-chain polyunsaturated fatty acids. Mutations in this gene have been associated with spinocerebellar ataxia-38 (SCA38). Alternatively spliced transcript variants have been found for this gene.
Genomic regions Chromosome 6
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ELOVL5-203 ENST00000370918.8 3081 326aa Protein coding CCDS56433 Q9NYP7-2 - TSL:2, GENCODE basic,
ELOVL5-207 ENST00000542638.5 2875 262aa Protein coding CCDS75470 A0A0A0MTI6 - TSL:1, GENCODE basic,
ELOVL5-201 ENST00000304434.11 2765 299aa Protein coding CCDS4951 Q9NYP7-1 NM_021814.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ELOVL5-202 ENST00000370913.5 580 88aa Protein coding CCDS56434 Q9NYP7-3 - TSL:1, GENCODE basic,
ELOVL5-206 ENST00000486973.1 794 No protein Processed transcript - - - TSL:3,
ELOVL5-205 ENST00000485336.5 761 No protein Processed transcript - - - TSL:5,
ELOVL5-204 ENST00000465983.5 696 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ELOVL5
This KO Strategy loading
Red Cotton™ Notes Gene ELOVL5 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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