CRISPR-U™ Gene Knockout Cell Line
Strategy
ELOVL5 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ELOVL5 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ELOVL5 |
| Gene id | 60481 |
| Organism | Homo sapiens |
| Official full symbol | ELOVL fatty acid elongase 5 |
| Gene type | protein-coding |
| Also known as | HELO1, SCA38, dJ483K16.1 |
| Summary | This gene belongs to the ELO family. It is highly expressed in the adrenal gland and testis, and encodes a multi-pass membrane protein that is localized in the endoplasmic reticulum. This protein is involved in the elongation of long-chain polyunsaturated fatty acids. Mutations in this gene have been associated with spinocerebellar ataxia-38 (SCA38). Alternatively spliced transcript variants have been found for this gene. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ELOVL5-203 | ENST00000370918.8 | 3081 | 326aa | Protein coding | CCDS56433 | Q9NYP7-2 | - | TSL:2, GENCODE basic, |
| ELOVL5-207 | ENST00000542638.5 | 2875 | 262aa | Protein coding | CCDS75470 | A0A0A0MTI6 | - | TSL:1, GENCODE basic, |
| ELOVL5-201 | ENST00000304434.11 | 2765 | 299aa | Protein coding | CCDS4951 | Q9NYP7-1 | NM_021814.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ELOVL5-202 | ENST00000370913.5 | 580 | 88aa | Protein coding | CCDS56434 | Q9NYP7-3 | - | TSL:1, GENCODE basic, |
| ELOVL5-206 | ENST00000486973.1 | 794 | No protein | Processed transcript | - | - | - | TSL:3, |
| ELOVL5-205 | ENST00000485336.5 | 761 | No protein | Processed transcript | - | - | - | TSL:5, |
| ELOVL5-204 | ENST00000465983.5 | 696 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ELOVL5 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ELOVL5 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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