CRISPR-U™ Gene Knockout Cell Line Strategy

BCL3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human BCL3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BCL3
Gene id 602
Organism Homo sapiens
Official full symbol BCL3 transcription coactivator
Gene type protein-coding
Also known as BCL4, D19S37
Summary This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B.
Genomic regions Chromosome 19
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BCL3-201 ENST00000164227.10 1877 454aa Protein coding CCDS12642 P20749 NM_005178.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
BCL3-203 ENST00000444487.1 923 308aa Protein coding - H7C0A2 - CDS 5' and 3' incomplete, TSL:5,
BCL3-209 ENST00000487394.1 603 No protein Processed transcript - - - TSL:3,
BCL3-205 ENST00000473468.1 587 No protein Processed transcript - - - TSL:5,
BCL3-202 ENST00000403534.7 1714 No protein Retained intron - - - TSL:2,
BCL3-207 ENST00000474300.1 819 No protein Retained intron - - - TSL:2,
BCL3-206 ENST00000473473.1 445 No protein Retained intron - - - TSL:2,
BCL3-204 ENST00000464319.1 439 No protein Retained intron - - - TSL:2,
BCL3-208 ENST00000477832.1 385 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BCL3
This KO Strategy loading
Red Cotton™ Notes Gene BCL3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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