CRISPR-U™ Gene Knockout Cell Line
Strategy
BCL3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BCL3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BCL3 |
| Gene id | 602 |
| Organism | Homo sapiens |
| Official full symbol | BCL3 transcription coactivator |
| Gene type | protein-coding |
| Also known as | BCL4, D19S37 |
| Summary | This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BCL3-201 | ENST00000164227.10 | 1877 | 454aa | Protein coding | CCDS12642 | P20749 | NM_005178.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BCL3-203 | ENST00000444487.1 | 923 | 308aa | Protein coding | - | H7C0A2 | - | CDS 5' and 3' incomplete, TSL:5, |
| BCL3-209 | ENST00000487394.1 | 603 | No protein | Processed transcript | - | - | - | TSL:3, |
| BCL3-205 | ENST00000473468.1 | 587 | No protein | Processed transcript | - | - | - | TSL:5, |
| BCL3-202 | ENST00000403534.7 | 1714 | No protein | Retained intron | - | - | - | TSL:2, |
| BCL3-207 | ENST00000474300.1 | 819 | No protein | Retained intron | - | - | - | TSL:2, |
| BCL3-206 | ENST00000473473.1 | 445 | No protein | Retained intron | - | - | - | TSL:2, |
| BCL3-204 | ENST00000464319.1 | 439 | No protein | Retained intron | - | - | - | TSL:2, |
| BCL3-208 | ENST00000477832.1 | 385 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BCL3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BCL3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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