CRISPR-U™ Gene Knockout Cell Line Strategy

BCL2L2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human BCL2L2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BCL2L2
Gene id 599
Organism Homo sapiens
Official full symbol BCL2 like 2
Gene type protein-coding
Also known as BCL-W, BCL2-L-2, BCLW, PPP1R51
Summary This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream PABPN1 (poly(A) binding protein, nuclear 1) gene.
Genomic regions Chromosome 14
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BCL2L2-208 ENST00000678311.1 3568 193aa Protein coding CCDS9591 - - GENCODE basic, APPRIS P1,
BCL2L2-210 ENST00000679219.1 3557 193aa Protein coding CCDS9591 - - GENCODE basic, APPRIS P1,
BCL2L2-209 ENST00000679000.1 3539 193aa Protein coding CCDS9591 - - GENCODE basic, APPRIS P1,
BCL2L2-201 ENST00000250405.10 3526 193aa Protein coding CCDS9591 Q92843-1 NM_004050.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
BCL2L2-206 ENST00000557236.6 1245 168aa Protein coding - G3V4B7 - TSL:5, GENCODE basic,
BCL2L2-207 ENST00000557579.2 762 185aa Protein coding - G3V5A9 - TSL:3, GENCODE basic,
BCL2L2-202 ENST00000553824.5 548 51aa Protein coding - G3V3B7 - CDS 3' incomplete, TSL:4,
BCL2L2-205 ENST00000556599.1 548 167aa Protein coding - H0YJC7 - CDS 5' incomplete, TSL:3,
BCL2L2-203 ENST00000554635.1 545 71aa Protein coding - G3V3G8 - CDS 3' incomplete, TSL:1,
BCL2L2-204 ENST00000556100.3 1368 193aa Nonsense mediated decay CCDS9591 - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BCL2L2
This KO Strategy loading
Red Cotton™ Notes Gene BCL2L2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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