CRISPR-U™ Gene Knockout Cell Line
Strategy
BCL2L2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BCL2L2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BCL2L2 |
| Gene id | 599 |
| Organism | Homo sapiens |
| Official full symbol | BCL2 like 2 |
| Gene type | protein-coding |
| Also known as | BCL-W, BCL2-L-2, BCLW, PPP1R51 |
| Summary | This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream PABPN1 (poly(A) binding protein, nuclear 1) gene. |
| Genomic regions | Chromosome 14 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BCL2L2-208 | ENST00000678311.1 | 3568 | 193aa | Protein coding | CCDS9591 | - | - | GENCODE basic, APPRIS P1, |
| BCL2L2-210 | ENST00000679219.1 | 3557 | 193aa | Protein coding | CCDS9591 | - | - | GENCODE basic, APPRIS P1, |
| BCL2L2-209 | ENST00000679000.1 | 3539 | 193aa | Protein coding | CCDS9591 | - | - | GENCODE basic, APPRIS P1, |
| BCL2L2-201 | ENST00000250405.10 | 3526 | 193aa | Protein coding | CCDS9591 | Q92843-1 | NM_004050.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BCL2L2-206 | ENST00000557236.6 | 1245 | 168aa | Protein coding | - | G3V4B7 | - | TSL:5, GENCODE basic, |
| BCL2L2-207 | ENST00000557579.2 | 762 | 185aa | Protein coding | - | G3V5A9 | - | TSL:3, GENCODE basic, |
| BCL2L2-202 | ENST00000553824.5 | 548 | 51aa | Protein coding | - | G3V3B7 | - | CDS 3' incomplete, TSL:4, |
| BCL2L2-205 | ENST00000556599.1 | 548 | 167aa | Protein coding | - | H0YJC7 | - | CDS 5' incomplete, TSL:3, |
| BCL2L2-203 | ENST00000554635.1 | 545 | 71aa | Protein coding | - | G3V3G8 | - | CDS 3' incomplete, TSL:1, |
| BCL2L2-204 | ENST00000556100.3 | 1368 | 193aa | Nonsense mediated decay | CCDS9591 | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BCL2L2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BCL2L2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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