CRISPR-U™ Gene Knockout Cell Line Strategy

RELB Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human RELB Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol RELB
Gene id 5971
Organism Homo sapiens
Official full symbol RELB proto-oncogene, NF-kB subunit
Gene type protein-coding
Also known as I-REL, IMD53, IREL, REL-B
Genomic regions Chromosome 19
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
RELB-201 ENST00000221452.13 2258 579aa Protein coding CCDS46110 Q01201 NM_006509.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
RELB-207 ENST00000625761.2 2279 579aa Protein coding - Q01201 - TSL:5, GENCODE basic, APPRIS P2,
RELB-202 ENST00000505236.1 2219 576aa Protein coding - D6R992 - TSL:5, GENCODE basic, APPRIS ALT2,
RELB-206 ENST00000589972.1 760 142aa Protein coding - K7ERX9 - CDS 5' incomplete, TSL:3,
RELB-204 ENST00000509480.5 840 58aa Nonsense mediated decay - D6RIV7 - TSL:3,
RELB-203 ENST00000509229.1 775 No protein Retained intron - - - TSL:5,
RELB-205 ENST00000510184.1 546 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene RELB
This KO Strategy loading
Red Cotton™ Notes Gene RELB had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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