CRISPR-U™ Gene Knockout Cell Line
Strategy
GNB4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GNB4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GNB4 |
| Gene id | 59345 |
| Organism | Homo sapiens |
| Official full symbol | G protein subunit beta 4 |
| Gene type | protein-coding |
| Also known as | CMTD1F |
| Summary | Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GNB4-210 | ENST00000676128.1 | 6386 | 340aa | Protein coding | CCDS3230 | - | - | GENCODE basic, APPRIS P1, |
| GNB4-201 | ENST00000232564.8 | 6315 | 340aa | Protein coding | CCDS3230 | Q9HAV0 | NM_021629.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GNB4-207 | ENST00000674862.1 | 1173 | 340aa | Protein coding | CCDS3230 | - | - | GENCODE basic, APPRIS P1, |
| GNB4-208 | ENST00000674927.1 | 1696 | 306aa | Protein coding | - | - | - | GENCODE basic, |
| GNB4-206 | ENST00000674713.1 | 1196 | 304aa | Protein coding | - | - | - | GENCODE basic, |
| GNB4-209 | ENST00000675901.1 | 1187 | 322aa | Protein coding | - | - | - | GENCODE basic, |
| GNB4-203 | ENST00000466899.6 | 1170 | 242aa | Protein coding | - | H7C5J5 | - | TSL:1, GENCODE basic, |
| GNB4-204 | ENST00000468623.6 | 984 | 327aa | Protein coding | - | Q9HAV0 | - | TSL:2, GENCODE basic, |
| GNB4-202 | ENST00000465153.2 | 978 | 245aa | Protein coding | - | - | - | TSL:3, GENCODE basic, |
| GNB4-205 | ENST00000497513.1 | 571 | 79aa | Protein coding | - | C9JD14 | - | CDS 3' incomplete, TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GNB4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GNB4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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