CRISPR-U™ Gene Knockout Cell Line Strategy

GNB4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GNB4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GNB4
Gene id 59345
Organism Homo sapiens
Official full symbol G protein subunit beta 4
Gene type protein-coding
Also known as CMTD1F
Summary Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.
Genomic regions Chromosome 3
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GNB4-210 ENST00000676128.1 6386 340aa Protein coding CCDS3230 - - GENCODE basic, APPRIS P1,
GNB4-201 ENST00000232564.8 6315 340aa Protein coding CCDS3230 Q9HAV0 NM_021629.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GNB4-207 ENST00000674862.1 1173 340aa Protein coding CCDS3230 - - GENCODE basic, APPRIS P1,
GNB4-208 ENST00000674927.1 1696 306aa Protein coding - - - GENCODE basic,
GNB4-206 ENST00000674713.1 1196 304aa Protein coding - - - GENCODE basic,
GNB4-209 ENST00000675901.1 1187 322aa Protein coding - - - GENCODE basic,
GNB4-203 ENST00000466899.6 1170 242aa Protein coding - H7C5J5 - TSL:1, GENCODE basic,
GNB4-204 ENST00000468623.6 984 327aa Protein coding - Q9HAV0 - TSL:2, GENCODE basic,
GNB4-202 ENST00000465153.2 978 245aa Protein coding - - - TSL:3, GENCODE basic,
GNB4-205 ENST00000497513.1 571 79aa Protein coding - C9JD14 - CDS 3' incomplete, TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GNB4
This KO Strategy loading
Red Cotton™ Notes Gene GNB4 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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