CRISPR-U™ Gene Knockout Cell Line Strategy

RANBP1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human RANBP1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol RANBP1
Gene id 5902
Organism Homo sapiens
Official full symbol RAN binding protein 1
Gene type protein-coding
Also known as HTF9A
Summary This gene encodes a protein that forms a complex with Ras-related nuclear protein (Ran) and metabolizes guanoside triphosphate (GTP). This complex participates in the regulation of the cell cycle by controlling transport of proteins and nucleic acids into the nucleus. There are multiple pseudogenes for this gene on chromosomes 9, 12, 17, and X. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 22
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
RANBP1-208 ENST00000430524.6 1221 278aa Protein coding CCDS74823 F6WQW2 NM_001278639.2 TSL:3, GENCODE basic, APPRIS ALT2, MANE Select v0.92,
RANBP1-202 ENST00000402752.5 1132 200aa Protein coding CCDS63408 P43487-2 - TSL:1, GENCODE basic, APPRIS ALT2,
RANBP1-201 ENST00000331821.7 837 201aa Protein coding CCDS13775 P43487-1 - TSL:1, GENCODE basic, APPRIS P4,
RANBP1-205 ENST00000418705.2 932 145aa Protein coding - C9JXG8 - CDS 3' incomplete, TSL:3,
RANBP1-207 ENST00000423859.5 832 204aa Protein coding - C9JGV6 - CDS 3' incomplete, TSL:3,
RANBP1-209 ENST00000432879.5 744 165aa Protein coding - C9JDM3 - CDS 3' incomplete, TSL:3,
RANBP1-204 ENST00000416427.5 645 53aa Protein coding - C9JIC6 - CDS 3' incomplete, TSL:5,
RANBP1-203 ENST00000411892.5 584 163aa Protein coding - C9JJ34 - CDS 3' incomplete, TSL:2,
RANBP1-206 ENST00000421656.5 333 53aa Protein coding - C9JIC6 - CDS 3' incomplete, TSL:3,
RANBP1-210 ENST00000435265.5 1879 73aa Nonsense mediated decay - F8WCY3 - TSL:2,
RANBP1-211 ENST00000448394.2 688 No protein Processed transcript - - - TSL:2,
RANBP1-212 ENST00000467920.1 529 No protein Processed transcript - - - TSL:2,
RANBP1-213 ENST00000486575.1 2177 No protein Retained intron - - - TSL:2,
RANBP1-214 ENST00000488484.1 699 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene RANBP1
This KO Strategy loading
Red Cotton™ Notes Gene RANBP1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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