CRISPR-U™ Gene Knockout Cell Line
Strategy
RANBP1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human RANBP1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | RANBP1 |
| Gene id | 5902 |
| Organism | Homo sapiens |
| Official full symbol | RAN binding protein 1 |
| Gene type | protein-coding |
| Also known as | HTF9A |
| Summary | This gene encodes a protein that forms a complex with Ras-related nuclear protein (Ran) and metabolizes guanoside triphosphate (GTP). This complex participates in the regulation of the cell cycle by controlling transport of proteins and nucleic acids into the nucleus. There are multiple pseudogenes for this gene on chromosomes 9, 12, 17, and X. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 22 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| RANBP1-208 | ENST00000430524.6 | 1221 | 278aa | Protein coding | CCDS74823 | F6WQW2 | NM_001278639.2 | TSL:3, GENCODE basic, APPRIS ALT2, MANE Select v0.92, |
| RANBP1-202 | ENST00000402752.5 | 1132 | 200aa | Protein coding | CCDS63408 | P43487-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| RANBP1-201 | ENST00000331821.7 | 837 | 201aa | Protein coding | CCDS13775 | P43487-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| RANBP1-205 | ENST00000418705.2 | 932 | 145aa | Protein coding | - | C9JXG8 | - | CDS 3' incomplete, TSL:3, |
| RANBP1-207 | ENST00000423859.5 | 832 | 204aa | Protein coding | - | C9JGV6 | - | CDS 3' incomplete, TSL:3, |
| RANBP1-209 | ENST00000432879.5 | 744 | 165aa | Protein coding | - | C9JDM3 | - | CDS 3' incomplete, TSL:3, |
| RANBP1-204 | ENST00000416427.5 | 645 | 53aa | Protein coding | - | C9JIC6 | - | CDS 3' incomplete, TSL:5, |
| RANBP1-203 | ENST00000411892.5 | 584 | 163aa | Protein coding | - | C9JJ34 | - | CDS 3' incomplete, TSL:2, |
| RANBP1-206 | ENST00000421656.5 | 333 | 53aa | Protein coding | - | C9JIC6 | - | CDS 3' incomplete, TSL:3, |
| RANBP1-210 | ENST00000435265.5 | 1879 | 73aa | Nonsense mediated decay | - | F8WCY3 | - | TSL:2, |
| RANBP1-211 | ENST00000448394.2 | 688 | No protein | Processed transcript | - | - | - | TSL:2, |
| RANBP1-212 | ENST00000467920.1 | 529 | No protein | Processed transcript | - | - | - | TSL:2, |
| RANBP1-213 | ENST00000486575.1 | 2177 | No protein | Retained intron | - | - | - | TSL:2, |
| RANBP1-214 | ENST00000488484.1 | 699 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | RANBP1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene RANBP1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: