CRISPR-U™ Gene Knockout Cell Line
Strategy
RALA Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human RALA Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | RALA |
| Gene id | 5898 |
| Organism | Homo sapiens |
| Official full symbol | RAS like proto-oncogene A |
| Gene type | protein-coding |
| Also known as | RAL |
| Summary | The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| RALA-201 | ENST00000005257.7 | 2787 | 206aa | Protein coding | CCDS5460 | P11233 | NM_005402.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| RALA-203 | ENST00000436179.1 | 882 | 101aa | Protein coding | - | C9JPE8 | - | CDS 3' incomplete, TSL:2, |
| RALA-202 | ENST00000434466.1 | 768 | 164aa | Nonsense mediated decay | - | H7C3P7 | - | CDS 5' incomplete, TSL:3, |
| RALA-205 | ENST00000468201.1 | 705 | No protein | Processed transcript | - | - | - | TSL:4, |
| RALA-204 | ENST00000466491.1 | 320 | No protein | Processed transcript | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | RALA |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene RALA had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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