CRISPR-U™ Gene Knockout Cell Line Strategy

RALA Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human RALA Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol RALA
Gene id 5898
Organism Homo sapiens
Official full symbol RAS like proto-oncogene A
Gene type protein-coding
Also known as RAL
Summary The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins.
Genomic regions Chromosome 7
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
RALA-201 ENST00000005257.7 2787 206aa Protein coding CCDS5460 P11233 NM_005402.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
RALA-203 ENST00000436179.1 882 101aa Protein coding - C9JPE8 - CDS 3' incomplete, TSL:2,
RALA-202 ENST00000434466.1 768 164aa Nonsense mediated decay - H7C3P7 - CDS 5' incomplete, TSL:3,
RALA-205 ENST00000468201.1 705 No protein Processed transcript - - - TSL:4,
RALA-204 ENST00000466491.1 320 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene RALA
This KO Strategy loading
Red Cotton™ Notes Gene RALA had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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