CRISPR-U™ Gene Knockout Cell Line
Strategy
RAF1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human RAF1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | RAF1 |
| Gene id | 5894 |
| Organism | Homo sapiens |
| Official full symbol | Raf-1 proto-oncogene, serine/threonine kinase |
| Gene type | protein-coding |
| Also known as | CMD1NN, CRAF, NS5, Raf-1, c-Raf |
| Summary | This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| RAF1-205 | ENST00000442415.7 | 3251 | 668aa | Protein coding | CCDS87047 | P04049-2 | NM_001354689.3 | TSL:5, GENCODE basic, APPRIS ALT1, MANE Select v0.92, |
| RAF1-201 | ENST00000251849.9 | 3191 | 648aa | Protein coding | CCDS2612 | P04049-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| RAF1-204 | ENST00000432427.2 | 1831 | 527aa | Protein coding | - | H7C155 | - | CDS 5' incomplete, TSL:5, |
| RAF1-203 | ENST00000423275.5 | 2354 | 69aa | Nonsense mediated decay | - | A0A0B4J1W9 | - | TSL:2, |
| RAF1-202 | ENST00000416093.1 | 962 | 69aa | Nonsense mediated decay | - | A0A0B4J1W9 | - | TSL:2, |
| RAF1-212 | ENST00000494557.1 | 3205 | No protein | Retained intron | - | - | - | TSL:1, |
| RAF1-207 | ENST00000465826.5 | 1518 | No protein | Retained intron | - | - | - | TSL:3, |
| RAF1-208 | ENST00000471449.1 | 804 | No protein | Retained intron | - | - | - | TSL:2, |
| RAF1-211 | ENST00000492690.1 | 633 | No protein | Retained intron | - | - | - | TSL:3, |
| RAF1-209 | ENST00000475353.1 | 599 | No protein | Retained intron | - | - | - | TSL:4, |
| RAF1-210 | ENST00000491290.1 | 572 | No protein | Retained intron | - | - | - | TSL:4, |
| RAF1-206 | ENST00000460610.1 | 484 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | RAF1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene RAF1 had been KO in hct116 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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