CRISPR-U™ Gene Knockout Cell Line Strategy

RAD23B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human RAD23B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol RAD23B
Gene id 5887
Organism Homo sapiens
Official full symbol RAD23 homolog B, nucleotide excision repair protein
Gene type protein-coding
Also known as HHR23B, HR23B, P58
Summary The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Genomic regions Chromosome 9
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
RAD23B-201 ENST00000358015.8 4114 409aa Protein coding CCDS6769 P54727-1 NM_002874.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
RAD23B-202 ENST00000416373.6 3835 337aa Protein coding CCDS59138 P54727-2 - TSL:1, GENCODE basic,
RAD23B-205 ENST00000457811.1 811 114aa Protein coding - H0Y579 - CDS 5' incomplete, TSL:3,
RAD23B-203 ENST00000419616.5 716 146aa Protein coding - Q5W0S5 - CDS 3' incomplete, TSL:3,
RAD23B-204 ENST00000442587.1 456 80aa Protein coding - Q5W0S4 - CDS 3' incomplete, TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene RAD23B
This KO Strategy loading
Red Cotton™ Notes Gene RAD23B had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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