CRISPR-U™ Gene Knockout Cell Line
Strategy
RAD23B Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human RAD23B Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | RAD23B |
| Gene id | 5887 |
| Organism | Homo sapiens |
| Official full symbol | RAD23 homolog B, nucleotide excision repair protein |
| Gene type | protein-coding |
| Also known as | HHR23B, HR23B, P58 |
| Summary | The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| RAD23B-201 | ENST00000358015.8 | 4114 | 409aa | Protein coding | CCDS6769 | P54727-1 | NM_002874.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| RAD23B-202 | ENST00000416373.6 | 3835 | 337aa | Protein coding | CCDS59138 | P54727-2 | - | TSL:1, GENCODE basic, |
| RAD23B-205 | ENST00000457811.1 | 811 | 114aa | Protein coding | - | H0Y579 | - | CDS 5' incomplete, TSL:3, |
| RAD23B-203 | ENST00000419616.5 | 716 | 146aa | Protein coding | - | Q5W0S5 | - | CDS 3' incomplete, TSL:3, |
| RAD23B-204 | ENST00000442587.1 | 456 | 80aa | Protein coding | - | Q5W0S4 | - | CDS 3' incomplete, TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | RAD23B |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene RAD23B had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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