CRISPR-U™ Gene Knockout Cell Line
Strategy
RAD23A Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human RAD23A Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | RAD23A |
| Gene id | 5886 |
| Organism | Homo sapiens |
| Official full symbol | RAD23 homolog A, nucleotide excision repair protein |
| Gene type | protein-coding |
| Also known as | HHR23A, HR23A |
| Summary | The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair. Proteins in this family have a modular domain structure consisting of an ubiquitin-like domain (UbL), ubiquitin-associated domain 1 (UbA1), XPC-binding domain and UbA2. The protein encoded by this gene plays an important role in nucleotide excision repair and also in delivery of polyubiquitinated proteins to the proteasome. Alternative splicing results in multiple transcript variants encoding multiple isoforms. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| RAD23A-203 | ENST00000586534.6 | 1772 | 363aa | Protein coding | CCDS12289 | P54725-1 | NM_005053.4 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| RAD23A-201 | ENST00000316856.7 | 1736 | 362aa | Protein coding | CCDS59358 | P54725-3 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| RAD23A-209 | ENST00000592268.5 | 1526 | 308aa | Protein coding | CCDS59357 | P54725-2 | - | TSL:5, GENCODE basic, |
| RAD23A-206 | ENST00000590881.1 | 999 | 150aa | Protein coding | - | K7EQ16 | - | CDS 5' incomplete, TSL:5, |
| RAD23A-211 | ENST00000651137.1 | 1871 | 282aa | Nonsense mediated decay | - | A0A494C0B4 | - | - |
| RAD23A-210 | ENST00000593114.5 | 1691 | 139aa | Nonsense mediated decay | - | K7ENJ0 | - | TSL:5, |
| RAD23A-208 | ENST00000591499.5 | 1350 | 142aa | Nonsense mediated decay | - | K7ELW1 | - | TSL:2, |
| RAD23A-202 | ENST00000586375.1 | 894 | 62aa | Nonsense mediated decay | - | K7ELU6 | - | CDS 5' incomplete, TSL:3, |
| RAD23A-205 | ENST00000588826.2 | 832 | No protein | Processed transcript | - | - | - | TSL:5, |
| RAD23A-204 | ENST00000588329.5 | 1034 | No protein | Retained intron | - | - | - | TSL:5, |
| RAD23A-207 | ENST00000591467.1 | 545 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | RAD23A |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene RAD23A had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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