CRISPR-U™ Gene Knockout Cell Line Strategy

RAD23A Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human RAD23A Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol RAD23A
Gene id 5886
Organism Homo sapiens
Official full symbol RAD23 homolog A, nucleotide excision repair protein
Gene type protein-coding
Also known as HHR23A, HR23A
Summary The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair. Proteins in this family have a modular domain structure consisting of an ubiquitin-like domain (UbL), ubiquitin-associated domain 1 (UbA1), XPC-binding domain and UbA2. The protein encoded by this gene plays an important role in nucleotide excision repair and also in delivery of polyubiquitinated proteins to the proteasome. Alternative splicing results in multiple transcript variants encoding multiple isoforms.
Genomic regions Chromosome 19
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
RAD23A-203 ENST00000586534.6 1772 363aa Protein coding CCDS12289 P54725-1 NM_005053.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
RAD23A-201 ENST00000316856.7 1736 362aa Protein coding CCDS59358 P54725-3 - TSL:1, GENCODE basic, APPRIS ALT1,
RAD23A-209 ENST00000592268.5 1526 308aa Protein coding CCDS59357 P54725-2 - TSL:5, GENCODE basic,
RAD23A-206 ENST00000590881.1 999 150aa Protein coding - K7EQ16 - CDS 5' incomplete, TSL:5,
RAD23A-211 ENST00000651137.1 1871 282aa Nonsense mediated decay - A0A494C0B4 - -
RAD23A-210 ENST00000593114.5 1691 139aa Nonsense mediated decay - K7ENJ0 - TSL:5,
RAD23A-208 ENST00000591499.5 1350 142aa Nonsense mediated decay - K7ELW1 - TSL:2,
RAD23A-202 ENST00000586375.1 894 62aa Nonsense mediated decay - K7ELU6 - CDS 5' incomplete, TSL:3,
RAD23A-205 ENST00000588826.2 832 No protein Processed transcript - - - TSL:5,
RAD23A-204 ENST00000588329.5 1034 No protein Retained intron - - - TSL:5,
RAD23A-207 ENST00000591467.1 545 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene RAD23A
This KO Strategy loading
Red Cotton™ Notes Gene RAD23A had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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