CRISPR-U™ Gene Knockout Cell Line Strategy

RAB13 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human RAB13 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol RAB13
Gene id 5872
Organism Homo sapiens
Official full symbol RAB13, member RAS oncogene family
Gene type protein-coding
Also known as GIG4
Summary This gene is a member of the Rab family of small G proteins and plays a role in regulating membrane trafficking between trans-Golgi network (TGN) and recycling endosomes (RE). The encoded protein is involved in the assembly of tight junctions, which are components of the apical junctional complex (AJC) of epithelial cells. The AJC plays a role in forming a barrier between luminal contents and the underlying tissue. Additional functions associated with the protein include endocytic recycling of occludin, regulation of epithelial cell scattering, neuronal regeneration and regulation of neurite outgrowth. Alternately spliced transcript variants have been observed for this gene. A pseudogene associated with this gene is located on chromosome 12.
Genomic regions Chromosome 1
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
RAB13-205 ENST00000614713.4 1353 122aa Protein coding CCDS72921 A0A087WWB9 - TSL:5, GENCODE basic,
RAB13-201 ENST00000368575.5 1164 203aa Protein coding CCDS1058 P51153 NM_002870.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
RAB13-202 ENST00000462680.5 2123 No protein Processed transcript - - - TSL:2,
RAB13-204 ENST00000495720.5 868 No protein Processed transcript - - - TSL:5,
RAB13-203 ENST00000484297.1 643 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene RAB13
This KO Strategy loading
Red Cotton™ Notes Gene RAB13 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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