CRISPR-U™ Gene Knockout Cell Line Strategy

RAB4A Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human RAB4A Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol RAB4A
Gene id 5867
Organism Homo sapiens
Official full symbol RAB4A, member RAS oncogene family
Gene type protein-coding
Also known as HRES-1, HRES-1/RAB4, HRES1, RAB4
Summary This gene is a member of the largest group in the Ras superfamily of small GTPases, which regulate membrane trafficking. The encoded protein is associated with early endosomes and is involved in their sorting and recycling. The protein also plays a role in regulating the recycling of receptors from endosomes to the plasma membrane. Alternatively spliced transcript variants have been observed for this gene.
Genomic regions Chromosome 1
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
RAB4A-201 ENST00000366690.5 2987 218aa Protein coding CCDS31050 A0A024R3U9 P20338 NM_004578.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
RAB4A-205 ENST00000618010.4 2799 113aa Protein coding CCDS73046 A0A087WYT5 - TSL:3, GENCODE basic,
RAB4A-202 ENST00000473894.1 708 No protein Processed transcript - - - TSL:3,
RAB4A-204 ENST00000489342.1 431 No protein Processed transcript - - - TSL:5,
RAB4A-203 ENST00000481981.1 531 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene RAB4A
This KO Strategy loading
Red Cotton™ Notes Gene RAB4A had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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