CRISPR-U™ Gene Knockout Cell Line Strategy

PXN Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PXN Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PXN
Gene id 5829
Organism Homo sapiens
Official full symbol paxillin
Gene type protein-coding
Summary This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445).
Genomic regions Chromosome 12
Strategy Summary
This gene has 11 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PXN-202 ENST00000267257.11 3835 605aa Protein coding CCDS58281 P49023-3 - TSL:5, GENCODE basic, APPRIS ALT2,
PXN-206 ENST00000458477.6 3807 424aa Protein coding CCDS44998 P49023-4 - TSL:1, GENCODE basic,
PXN-201 ENST00000228307.11 3785 591aa Protein coding CCDS44997 P49023-1 - TSL:1, GENCODE basic, APPRIS P4,
PXN-204 ENST00000424649.6 3683 557aa Protein coding CCDS44996 P49023-2 - TSL:1, GENCODE basic, APPRIS ALT2,
PXN-221 ENST00000637617.2 5214 1081aa Protein coding - A0A1B0GTU4 NM_001385981.1 TSL:5, GENCODE basic, APPRIS ALT2, MANE Select v0.92,
PXN-207 ENST00000536957.5 4112 589aa Protein coding - F5GZ78 - TSL:2, GENCODE basic, APPRIS ALT2,
PXN-210 ENST00000543331.1 957 176aa Protein coding - F5H836 - CDS 3' incomplete, TSL:4,
PXN-211 ENST00000546532.5 729 37aa Protein coding - F8W0K8 - CDS 3' incomplete, TSL:5,
PXN-215 ENST00000548912.5 655 36aa Protein coding - F8W0G0 - CDS 3' incomplete, TSL:5,
PXN-220 ENST00000637386.1 621 207aa Protein coding - A0A1B0GV30 - CDS 5' and 3' incomplete, TSL:5,
PXN-219 ENST00000552550.5 588 31aa Protein coding - F8W1E0 - CDS 3' incomplete, TSL:2,
PXN-203 ENST00000323871.9 5457 706aa Nonsense mediated decay - A0A1B0GU60 - TSL:2,
PXN-222 ENST00000637624.1 4132 192aa Nonsense mediated decay - A0A1B0GWE7 - CDS 5' incomplete, TSL:2,
PXN-214 ENST00000547983.5 1004 91aa Nonsense mediated decay - F8VZ39 - TSL:5,
PXN-208 ENST00000538144.5 1805 No protein Processed transcript - - - TSL:1,
PXN-218 ENST00000551327.5 588 No protein Processed transcript - - - TSL:4,
PXN-213 ENST00000547772.1 584 No protein Processed transcript - - - TSL:2,
PXN-212 ENST00000547746.1 579 No protein Processed transcript - - - TSL:4,
PXN-209 ENST00000540221.1 550 No protein Processed transcript - - - TSL:4,
PXN-216 ENST00000550205.1 547 No protein Processed transcript - - - TSL:4,
PXN-205 ENST00000440827.3 516 No protein Processed transcript - - - TSL:4,
PXN-217 ENST00000550795.1 513 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PXN
This KO Strategy loading
Red Cotton™ Notes Gene PXN had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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