CRISPR-U™ Gene Knockout Cell Line
Strategy
PXN Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PXN Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PXN |
| Gene id | 5829 |
| Organism | Homo sapiens |
| Official full symbol | paxillin |
| Gene type | protein-coding |
| Summary | This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 11 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PXN-202 | ENST00000267257.11 | 3835 | 605aa | Protein coding | CCDS58281 | P49023-3 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| PXN-206 | ENST00000458477.6 | 3807 | 424aa | Protein coding | CCDS44998 | P49023-4 | - | TSL:1, GENCODE basic, |
| PXN-201 | ENST00000228307.11 | 3785 | 591aa | Protein coding | CCDS44997 | P49023-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| PXN-204 | ENST00000424649.6 | 3683 | 557aa | Protein coding | CCDS44996 | P49023-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| PXN-221 | ENST00000637617.2 | 5214 | 1081aa | Protein coding | - | A0A1B0GTU4 | NM_001385981.1 | TSL:5, GENCODE basic, APPRIS ALT2, MANE Select v0.92, |
| PXN-207 | ENST00000536957.5 | 4112 | 589aa | Protein coding | - | F5GZ78 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| PXN-210 | ENST00000543331.1 | 957 | 176aa | Protein coding | - | F5H836 | - | CDS 3' incomplete, TSL:4, |
| PXN-211 | ENST00000546532.5 | 729 | 37aa | Protein coding | - | F8W0K8 | - | CDS 3' incomplete, TSL:5, |
| PXN-215 | ENST00000548912.5 | 655 | 36aa | Protein coding | - | F8W0G0 | - | CDS 3' incomplete, TSL:5, |
| PXN-220 | ENST00000637386.1 | 621 | 207aa | Protein coding | - | A0A1B0GV30 | - | CDS 5' and 3' incomplete, TSL:5, |
| PXN-219 | ENST00000552550.5 | 588 | 31aa | Protein coding | - | F8W1E0 | - | CDS 3' incomplete, TSL:2, |
| PXN-203 | ENST00000323871.9 | 5457 | 706aa | Nonsense mediated decay | - | A0A1B0GU60 | - | TSL:2, |
| PXN-222 | ENST00000637624.1 | 4132 | 192aa | Nonsense mediated decay | - | A0A1B0GWE7 | - | CDS 5' incomplete, TSL:2, |
| PXN-214 | ENST00000547983.5 | 1004 | 91aa | Nonsense mediated decay | - | F8VZ39 | - | TSL:5, |
| PXN-208 | ENST00000538144.5 | 1805 | No protein | Processed transcript | - | - | - | TSL:1, |
| PXN-218 | ENST00000551327.5 | 588 | No protein | Processed transcript | - | - | - | TSL:4, |
| PXN-213 | ENST00000547772.1 | 584 | No protein | Processed transcript | - | - | - | TSL:2, |
| PXN-212 | ENST00000547746.1 | 579 | No protein | Processed transcript | - | - | - | TSL:4, |
| PXN-209 | ENST00000540221.1 | 550 | No protein | Processed transcript | - | - | - | TSL:4, |
| PXN-216 | ENST00000550205.1 | 547 | No protein | Processed transcript | - | - | - | TSL:4, |
| PXN-205 | ENST00000440827.3 | 516 | No protein | Processed transcript | - | - | - | TSL:4, |
| PXN-217 | ENST00000550795.1 | 513 | No protein | Processed transcript | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PXN |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PXN had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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