CRISPR-U™ Gene Knockout Cell Line Strategy

PVR Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PVR Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PVR
Gene id 5817
Organism Homo sapiens
Official full symbol PVR cell adhesion molecule
Gene type protein-coding
Also known as CD155, HVED, NECL5, Necl-5, PVS, TAGE4
Summary The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 19
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PVR-205 ENST00000425690.8 5792 417aa Protein coding CCDS12640 A0A0C4DG49 NM_006505.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PVR-202 ENST00000344956.8 3166 364aa Protein coding CCDS46106 P15151-3 - TSL:5, GENCODE basic,
PVR-203 ENST00000403059.8 3078 372aa Protein coding CCDS46105 P15151-2 - TSL:2, GENCODE basic,
PVR-204 ENST00000406449.8 1344 392aa Protein coding CCDS46107 A0A0A0MSA9 - TSL:1, GENCODE basic,
PVR-206 ENST00000587785.1 356 70aa Protein coding - K7EMC6 - CDS 5' incomplete, TSL:3,
PVR-201 ENST00000187830.2 3132 44aa Nonsense mediated decay - F8W7D4 - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PVR
This KO Strategy loading
Red Cotton™ Notes Gene PVR had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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