CRISPR-U™ Gene Knockout Cell Line
Strategy
PVR Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PVR Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PVR |
| Gene id | 5817 |
| Organism | Homo sapiens |
| Official full symbol | PVR cell adhesion molecule |
| Gene type | protein-coding |
| Also known as | CD155, HVED, NECL5, Necl-5, PVS, TAGE4 |
| Summary | The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PVR-205 | ENST00000425690.8 | 5792 | 417aa | Protein coding | CCDS12640 | A0A0C4DG49 | NM_006505.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PVR-202 | ENST00000344956.8 | 3166 | 364aa | Protein coding | CCDS46106 | P15151-3 | - | TSL:5, GENCODE basic, |
| PVR-203 | ENST00000403059.8 | 3078 | 372aa | Protein coding | CCDS46105 | P15151-2 | - | TSL:2, GENCODE basic, |
| PVR-204 | ENST00000406449.8 | 1344 | 392aa | Protein coding | CCDS46107 | A0A0A0MSA9 | - | TSL:1, GENCODE basic, |
| PVR-206 | ENST00000587785.1 | 356 | 70aa | Protein coding | - | K7EMC6 | - | CDS 5' incomplete, TSL:3, |
| PVR-201 | ENST00000187830.2 | 3132 | 44aa | Nonsense mediated decay | - | F8W7D4 | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PVR |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PVR had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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