CRISPR-U™ Gene Knockout Cell Line Strategy

PTPRS Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PTPRS Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PTPRS
Gene id 5802
Organism Homo sapiens
Official full symbol protein tyrosine phosphatase receptor type S
Gene type protein-coding
Also known as PTPSIGMA, R-PTP-S, R-PTP-sigma
Summary The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular region, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this protein is composed of multiple Ig-like and fibronectin type III-like domains. Studies of the similar gene in mice suggested that this PTP may be involved in cell-cell interaction, primary axonogenesis, and axon guidance during embryogenesis. This PTP has been also implicated in the molecular control of adult nerve repair. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported.
Genomic regions Chromosome 19
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PTPRS-203 ENST00000587303.5 6353 1948aa Protein coding CCDS45930 Q13332-1 - TSL:1, GENCODE basic, APPRIS P1,
PTPRS-204 ENST00000588012.5 5993 1910aa Protein coding CCDS12140 Q13332-6 - TSL:1, GENCODE basic,
PTPRS-209 ENST00000592099.5 4766 1501aa Protein coding CCDS12139 Q13332-7 - TSL:1, GENCODE basic,
PTPRS-201 ENST00000262963.10 4766 1501aa Protein coding - G8JL96 - TSL:5, GENCODE basic,
PTPRS-207 ENST00000590509.5 893 128aa Protein coding - Q8NHS7 - TSL:1, GENCODE basic,
PTPRS-208 ENST00000591760.5 478 90aa Protein coding - K7ESP0 - CDS 3' incomplete, TSL:2,
PTPRS-202 ENST00000586065.1 388 79aa Protein coding - K7ERX4 - CDS 3' incomplete, TSL:5,
PTPRS-205 ENST00000588552.5 5443 No protein Processed transcript - - - TSL:1,
PTPRS-206 ENST00000589851.1 1270 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PTPRS
This KO Strategy loading
Red Cotton™ Notes Gene PTPRS had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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