CRISPR-U™ Gene Knockout Cell Line
Strategy
PTPRS Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PTPRS Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PTPRS |
| Gene id | 5802 |
| Organism | Homo sapiens |
| Official full symbol | protein tyrosine phosphatase receptor type S |
| Gene type | protein-coding |
| Also known as | PTPSIGMA, R-PTP-S, R-PTP-sigma |
| Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular region, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this protein is composed of multiple Ig-like and fibronectin type III-like domains. Studies of the similar gene in mice suggested that this PTP may be involved in cell-cell interaction, primary axonogenesis, and axon guidance during embryogenesis. This PTP has been also implicated in the molecular control of adult nerve repair. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PTPRS-203 | ENST00000587303.5 | 6353 | 1948aa | Protein coding | CCDS45930 | Q13332-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| PTPRS-204 | ENST00000588012.5 | 5993 | 1910aa | Protein coding | CCDS12140 | Q13332-6 | - | TSL:1, GENCODE basic, |
| PTPRS-209 | ENST00000592099.5 | 4766 | 1501aa | Protein coding | CCDS12139 | Q13332-7 | - | TSL:1, GENCODE basic, |
| PTPRS-201 | ENST00000262963.10 | 4766 | 1501aa | Protein coding | - | G8JL96 | - | TSL:5, GENCODE basic, |
| PTPRS-207 | ENST00000590509.5 | 893 | 128aa | Protein coding | - | Q8NHS7 | - | TSL:1, GENCODE basic, |
| PTPRS-208 | ENST00000591760.5 | 478 | 90aa | Protein coding | - | K7ESP0 | - | CDS 3' incomplete, TSL:2, |
| PTPRS-202 | ENST00000586065.1 | 388 | 79aa | Protein coding | - | K7ERX4 | - | CDS 3' incomplete, TSL:5, |
| PTPRS-205 | ENST00000588552.5 | 5443 | No protein | Processed transcript | - | - | - | TSL:1, |
| PTPRS-206 | ENST00000589851.1 | 1270 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PTPRS |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PTPRS had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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