CRISPR-U™ Gene Knockout Cell Line
Strategy
PTPRK Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PTPRK Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PTPRK |
| Gene id | 5796 |
| Organism | Homo sapiens |
| Official full symbol | protein tyrosine phosphatase receptor type K |
| Gene type | protein-coding |
| Also known as | R-PTP-kappa |
| Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP mu (MAM) domain, an Ig-like domain and four fibronectin type III-like repeats. This PTP was shown to mediate homophilic intercellular interaction, possibly through the interaction with beta- and gamma-catenin at adherens junctions. Expression of this gene was found to be stimulated by TGF-beta 1, which may be important for the inhibition of keratinocyte proliferation. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PTPRK-207 | ENST00000368226.9 | 6006 | 1440aa | Protein coding | CCDS5137 | Q15262-2 | NM_002844.4 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| PTPRK-205 | ENST00000368213.9 | 5816 | 1446aa | Protein coding | CCDS47473 | Q15262-3 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| PTPRK-221 | ENST00000532331.5 | 5757 | 1462aa | Protein coding | CCDS78179 | Q15262-4 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| PTPRK-206 | ENST00000368215.7 | 4651 | 1439aa | Protein coding | CCDS75517 | Q15262-1 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| PTPRK-204 | ENST00000368210.7 | 5025 | 1458aa | Protein coding | - | Q5TG12 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| PTPRK-203 | ENST00000368207.7 | 4758 | 1472aa | Protein coding | - | E9PGC5 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| PTPRK-218 | ENST00000525459.1 | 1722 | 248aa | Protein coding | - | E9PRW2 | - | TSL:2, GENCODE basic, |
| PTPRK-209 | ENST00000415046.6 | 1029 | 343aa | Protein coding | - | H0Y6P5 | - | CDS 5' and 3' incomplete, TSL:5, |
| PTPRK-210 | ENST00000415055.2 | 782 | 260aa | Protein coding | - | H0Y6V4 | - | CDS 5' and 3' incomplete, TSL:5, |
| PTPRK-213 | ENST00000490332.2 | 612 | 204aa | Protein coding | - | H0YDP9 | - | CDS 5' and 3' incomplete, TSL:5, |
| PTPRK-219 | ENST00000531050.5 | 2515 | 20aa | Nonsense mediated decay | - | H0YD44 | - | CDS 5' incomplete, TSL:1, |
| PTPRK-208 | ENST00000392449.4 | 1456 | 77aa | Nonsense mediated decay | - | Q6P493 | - | TSL:2, |
| PTPRK-222 | ENST00000532751.1 | 563 | 39aa | Nonsense mediated decay | - | E9PMV4 | - | TSL:4, |
| PTPRK-216 | ENST00000524481.5 | 3157 | No protein | Processed transcript | - | - | - | TSL:2, |
| PTPRK-217 | ENST00000524534.5 | 2441 | No protein | Processed transcript | - | - | - | TSL:5, |
| PTPRK-214 | ENST00000495748.5 | 746 | No protein | Processed transcript | - | - | - | TSL:5, |
| PTPRK-202 | ENST00000368205.7 | 662 | No protein | Processed transcript | - | - | - | TSL:2, |
| PTPRK-211 | ENST00000429595.1 | 558 | No protein | Processed transcript | - | - | - | TSL:3, |
| PTPRK-212 | ENST00000434424.5 | 555 | No protein | Processed transcript | - | - | - | TSL:3, |
| PTPRK-201 | ENST00000368202.4 | 526 | No protein | Processed transcript | - | - | - | TSL:2, |
| PTPRK-215 | ENST00000498284.1 | 524 | No protein | Processed transcript | - | - | - | TSL:5, |
| PTPRK-220 | ENST00000531466.1 | 311 | No protein | Retained intron | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PTPRK |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PTPRK had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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