CRISPR-U™ Gene Knockout Cell Line Strategy

PTPRK Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PTPRK Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PTPRK
Gene id 5796
Organism Homo sapiens
Official full symbol protein tyrosine phosphatase receptor type K
Gene type protein-coding
Also known as R-PTP-kappa
Summary The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP mu (MAM) domain, an Ig-like domain and four fibronectin type III-like repeats. This PTP was shown to mediate homophilic intercellular interaction, possibly through the interaction with beta- and gamma-catenin at adherens junctions. Expression of this gene was found to be stimulated by TGF-beta 1, which may be important for the inhibition of keratinocyte proliferation.
Genomic regions Chromosome 6
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PTPRK-207 ENST00000368226.9 6006 1440aa Protein coding CCDS5137 Q15262-2 NM_002844.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
PTPRK-205 ENST00000368213.9 5816 1446aa Protein coding CCDS47473 Q15262-3 - TSL:1, GENCODE basic, APPRIS ALT2,
PTPRK-221 ENST00000532331.5 5757 1462aa Protein coding CCDS78179 Q15262-4 - TSL:1, GENCODE basic, APPRIS ALT2,
PTPRK-206 ENST00000368215.7 4651 1439aa Protein coding CCDS75517 Q15262-1 - TSL:1, GENCODE basic, APPRIS ALT1,
PTPRK-204 ENST00000368210.7 5025 1458aa Protein coding - Q5TG12 - TSL:5, GENCODE basic, APPRIS ALT2,
PTPRK-203 ENST00000368207.7 4758 1472aa Protein coding - E9PGC5 - TSL:5, GENCODE basic, APPRIS ALT2,
PTPRK-218 ENST00000525459.1 1722 248aa Protein coding - E9PRW2 - TSL:2, GENCODE basic,
PTPRK-209 ENST00000415046.6 1029 343aa Protein coding - H0Y6P5 - CDS 5' and 3' incomplete, TSL:5,
PTPRK-210 ENST00000415055.2 782 260aa Protein coding - H0Y6V4 - CDS 5' and 3' incomplete, TSL:5,
PTPRK-213 ENST00000490332.2 612 204aa Protein coding - H0YDP9 - CDS 5' and 3' incomplete, TSL:5,
PTPRK-219 ENST00000531050.5 2515 20aa Nonsense mediated decay - H0YD44 - CDS 5' incomplete, TSL:1,
PTPRK-208 ENST00000392449.4 1456 77aa Nonsense mediated decay - Q6P493 - TSL:2,
PTPRK-222 ENST00000532751.1 563 39aa Nonsense mediated decay - E9PMV4 - TSL:4,
PTPRK-216 ENST00000524481.5 3157 No protein Processed transcript - - - TSL:2,
PTPRK-217 ENST00000524534.5 2441 No protein Processed transcript - - - TSL:5,
PTPRK-214 ENST00000495748.5 746 No protein Processed transcript - - - TSL:5,
PTPRK-202 ENST00000368205.7 662 No protein Processed transcript - - - TSL:2,
PTPRK-211 ENST00000429595.1 558 No protein Processed transcript - - - TSL:3,
PTPRK-212 ENST00000434424.5 555 No protein Processed transcript - - - TSL:3,
PTPRK-201 ENST00000368202.4 526 No protein Processed transcript - - - TSL:2,
PTPRK-215 ENST00000498284.1 524 No protein Processed transcript - - - TSL:5,
PTPRK-220 ENST00000531466.1 311 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PTPRK
This KO Strategy loading
Red Cotton™ Notes Gene PTPRK had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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