CRISPR-U™ Gene Knockout Cell Line Strategy

PTPRF Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PTPRF Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PTPRF
Gene id 5792
Organism Homo sapiens
Official full symbol protein tyrosine phosphatase receptor type F
Gene type protein-coding
Also known as BNAH2, LAR
Summary The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains three Ig-like domains, and nine non-Ig like domains similar to that of neural-cell adhesion molecule. This PTP was shown to function in the regulation of epithelial cell-cell contacts at adherents junctions, as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin-responsive tissue of obese, insulin-resistant individuals, and may contribute to the pathogenesis of insulin resistance. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.
Genomic regions Chromosome 1
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PTPRF-201 ENST00000359947.9 7720 1907aa Protein coding CCDS489 P10586-1 NM_002840.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
PTPRF-209 ENST00000438120.5 6377 1898aa Protein coding CCDS490 P10586-2 - TSL:1, GENCODE basic, APPRIS ALT1,
PTPRF-206 ENST00000429895.5 6312 1553aa Protein coding - H0Y6Z7 - CDS 5' incomplete, TSL:1,
PTPRF-205 ENST00000414879.5 4836 1332aa Protein coding - H0Y7Z9 - CDS 5' incomplete, TSL:2,
PTPRF-203 ENST00000372407.4 4600 979aa Protein coding - H0Y380 - CDS 5' incomplete, TSL:2,
PTPRF-204 ENST00000412568.6 4154 1291aa Protein coding - H0Y4H1 - CDS 5' incomplete, TSL:5,
PTPRF-216 ENST00000617451.4 1129 262aa Protein coding - A2A437 - TSL:5, GENCODE basic,
PTPRF-208 ENST00000437607.1 788 262aa Protein coding - A2A437 - CDS 3' incomplete, TSL:1,
PTPRF-215 ENST00000496447.5 5887 No protein Processed transcript - - - TSL:1,
PTPRF-212 ENST00000477970.1 3321 No protein Processed transcript - - - TSL:2,
PTPRF-207 ENST00000436724.5 2168 No protein Processed transcript - - - TSL:1,
PTPRF-202 ENST00000372405.1 1962 No protein Processed transcript - - - TSL:1,
PTPRF-211 ENST00000467464.1 571 No protein Processed transcript - - - TSL:3,
PTPRF-213 ENST00000481019.5 459 No protein Processed transcript - - - TSL:3,
PTPRF-214 ENST00000496043.5 415 No protein Processed transcript - - - TSL:5,
PTPRF-210 ENST00000463041.1 298 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PTPRF
This KO Strategy loading
Red Cotton™ Notes Gene PTPRF had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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