CRISPR-U™ Gene Knockout Cell Line
Strategy
PTPRF Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PTPRF Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PTPRF |
| Gene id | 5792 |
| Organism | Homo sapiens |
| Official full symbol | protein tyrosine phosphatase receptor type F |
| Gene type | protein-coding |
| Also known as | BNAH2, LAR |
| Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains three Ig-like domains, and nine non-Ig like domains similar to that of neural-cell adhesion molecule. This PTP was shown to function in the regulation of epithelial cell-cell contacts at adherents junctions, as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin-responsive tissue of obese, insulin-resistant individuals, and may contribute to the pathogenesis of insulin resistance. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PTPRF-201 | ENST00000359947.9 | 7720 | 1907aa | Protein coding | CCDS489 | P10586-1 | NM_002840.5 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| PTPRF-209 | ENST00000438120.5 | 6377 | 1898aa | Protein coding | CCDS490 | P10586-2 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| PTPRF-206 | ENST00000429895.5 | 6312 | 1553aa | Protein coding | - | H0Y6Z7 | - | CDS 5' incomplete, TSL:1, |
| PTPRF-205 | ENST00000414879.5 | 4836 | 1332aa | Protein coding | - | H0Y7Z9 | - | CDS 5' incomplete, TSL:2, |
| PTPRF-203 | ENST00000372407.4 | 4600 | 979aa | Protein coding | - | H0Y380 | - | CDS 5' incomplete, TSL:2, |
| PTPRF-204 | ENST00000412568.6 | 4154 | 1291aa | Protein coding | - | H0Y4H1 | - | CDS 5' incomplete, TSL:5, |
| PTPRF-216 | ENST00000617451.4 | 1129 | 262aa | Protein coding | - | A2A437 | - | TSL:5, GENCODE basic, |
| PTPRF-208 | ENST00000437607.1 | 788 | 262aa | Protein coding | - | A2A437 | - | CDS 3' incomplete, TSL:1, |
| PTPRF-215 | ENST00000496447.5 | 5887 | No protein | Processed transcript | - | - | - | TSL:1, |
| PTPRF-212 | ENST00000477970.1 | 3321 | No protein | Processed transcript | - | - | - | TSL:2, |
| PTPRF-207 | ENST00000436724.5 | 2168 | No protein | Processed transcript | - | - | - | TSL:1, |
| PTPRF-202 | ENST00000372405.1 | 1962 | No protein | Processed transcript | - | - | - | TSL:1, |
| PTPRF-211 | ENST00000467464.1 | 571 | No protein | Processed transcript | - | - | - | TSL:3, |
| PTPRF-213 | ENST00000481019.5 | 459 | No protein | Processed transcript | - | - | - | TSL:3, |
| PTPRF-214 | ENST00000496043.5 | 415 | No protein | Processed transcript | - | - | - | TSL:5, |
| PTPRF-210 | ENST00000463041.1 | 298 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PTPRF |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PTPRF had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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