CRISPR-U™ Gene Knockout Cell Line
Strategy
PTPN11 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PTPN11 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PTPN11 |
| Gene id | 5781 |
| Organism | Homo sapiens |
| Official full symbol | protein tyrosine phosphatase non-receptor type 11 |
| Gene type | protein-coding |
| Also known as | BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2 |
| Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PTPN11-201 | ENST00000351677.7 | 6073 | 593aa | Protein coding | CCDS9163 | Q06124-2 | NM_002834.5 | TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92, |
| PTPN11-202 | ENST00000392597.5 | 1876 | 460aa | Protein coding | CCDS58280 | Q06124-3 | - | TSL:1, GENCODE basic, |
| PTPN11-205 | ENST00000635625.1 | 1794 | 597aa | Protein coding | CCDS81741 | Q06124-1 | - | TSL:5, GENCODE basic, APPRIS P3, |
| PTPN11-207 | ENST00000639857.1 | 801 | 216aa | Protein coding | - | A0A1W2PPU4 | - | TSL:5, GENCODE basic, |
| PTPN11-206 | ENST00000635652.1 | 588 | 196aa | Protein coding | - | A0A0U1RRI0 | - | CDS 5' and 3' incomplete, TSL:3, |
| PTPN11-203 | ENST00000530818.1 | 486 | 108aa | Protein coding | - | H0YF12 | - | CDS 5' incomplete, TSL:3, |
| PTPN11-204 | ENST00000531326.1 | 573 | No protein | Processed transcript | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PTPN11 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PTPN11 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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