CRISPR-U™ Gene Knockout Cell Line Strategy

PTPN11 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PTPN11 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PTPN11
Gene id 5781
Organism Homo sapiens
Official full symbol protein tyrosine phosphatase non-receptor type 11
Gene type protein-coding
Also known as BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2
Summary The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.
Genomic regions Chromosome 12
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PTPN11-201 ENST00000351677.7 6073 593aa Protein coding CCDS9163 Q06124-2 NM_002834.5 TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92,
PTPN11-202 ENST00000392597.5 1876 460aa Protein coding CCDS58280 Q06124-3 - TSL:1, GENCODE basic,
PTPN11-205 ENST00000635625.1 1794 597aa Protein coding CCDS81741 Q06124-1 - TSL:5, GENCODE basic, APPRIS P3,
PTPN11-207 ENST00000639857.1 801 216aa Protein coding - A0A1W2PPU4 - TSL:5, GENCODE basic,
PTPN11-206 ENST00000635652.1 588 196aa Protein coding - A0A0U1RRI0 - CDS 5' and 3' incomplete, TSL:3,
PTPN11-203 ENST00000530818.1 486 108aa Protein coding - H0YF12 - CDS 5' incomplete, TSL:3,
PTPN11-204 ENST00000531326.1 573 No protein Processed transcript - - - TSL:4,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PTPN11
This KO Strategy loading
Red Cotton™ Notes Gene PTPN11 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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