CRISPR-U™ Gene Knockout Cell Line
Strategy
HES4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HES4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HES4 |
| Gene id | 57801 |
| Organism | Homo sapiens |
| Official full symbol | hes family bHLH transcription factor 4 |
| Gene type | protein-coding |
| Also known as | bHLHb42 |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HES4-202 | ENST00000428771.6 | 1040 | 247aa | Protein coding | CCDS44034 | E9PB28 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| HES4-201 | ENST00000304952.11 | 885 | 221aa | Protein coding | CCDS5 | Q9HCC6 | NM_021170.4 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| HES4-204 | ENST00000484667.2 | 667 | 189aa | Protein coding | - | D6REB3 | - | TSL:3, GENCODE basic, |
| HES4-203 | ENST00000481869.1 | 1038 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HES4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HES4 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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