CRISPR-U™ Gene Knockout Cell Line Strategy

HES4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HES4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HES4
Gene id 57801
Organism Homo sapiens
Official full symbol hes family bHLH transcription factor 4
Gene type protein-coding
Also known as bHLHb42
Genomic regions Chromosome 1
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HES4-202 ENST00000428771.6 1040 247aa Protein coding CCDS44034 E9PB28 - TSL:2, GENCODE basic, APPRIS ALT2,
HES4-201 ENST00000304952.11 885 221aa Protein coding CCDS5 Q9HCC6 NM_021170.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
HES4-204 ENST00000484667.2 667 189aa Protein coding - D6REB3 - TSL:3, GENCODE basic,
HES4-203 ENST00000481869.1 1038 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HES4
This KO Strategy loading
Red Cotton™ Notes Gene HES4 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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