CRISPR-U™ Gene Knockout Cell Line Strategy

PTPN6 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PTPN6 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PTPN6
Gene id 5777
Organism Homo sapiens
Official full symbol protein tyrosine phosphatase non-receptor type 6
Gene type protein-coding
Also known as HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L, SHP1
Summary The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported.
Genomic regions Chromosome 12
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PTPN6-204 ENST00000456013.5 2389 624aa Protein coding CCDS44821 P29350-4 - TSL:1, GENCODE basic,
PTPN6-201 ENST00000318974.14 2161 595aa Protein coding CCDS44820 P29350-1 NM_002831.6 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
PTPN6-202 ENST00000399448.5 2159 597aa Protein coding CCDS41744 P29350-3 - TSL:1, GENCODE basic, APPRIS ALT1,
PTPN6-220 ENST00000543115.5 650 130aa Protein coding - F5H5H9 - CDS 3' incomplete, TSL:4,
PTPN6-215 ENST00000541698.5 592 150aa Protein coding - F5H1Z8 - CDS 3' incomplete, TSL:4,
PTPN6-211 ENST00000538715.5 579 165aa Protein coding - F5H0N8 - CDS 3' incomplete, TSL:4,
PTPN6-217 ENST00000542462.1 570 144aa Protein coding - F5GY79 - CDS 3' incomplete, TSL:4,
PTPN6-223 ENST00000545153.5 959 72aa Nonsense mediated decay - U3KQS1 - TSL:3,
PTPN6-219 ENST00000542848.5 656 58aa Nonsense mediated decay - F5H4Z1 - TSL:5,
PTPN6-207 ENST00000536013.5 602 56aa Nonsense mediated decay - F5H1V7 - TSL:5,
PTPN6-206 ENST00000535462.5 543 60aa Nonsense mediated decay - F5GXD4 - TSL:4,
PTPN6-212 ENST00000539029.5 705 No protein Processed transcript - - - TSL:3,
PTPN6-209 ENST00000537533.1 673 No protein Processed transcript - - - TSL:3,
PTPN6-216 ENST00000542277.5 664 No protein Processed transcript - - - TSL:3,
PTPN6-214 ENST00000540740.5 592 No protein Processed transcript - - - TSL:4,
PTPN6-221 ENST00000543120.5 564 No protein Processed transcript - - - TSL:4,
PTPN6-205 ENST00000534900.5 538 No protein Processed transcript - - - TSL:4,
PTPN6-203 ENST00000416215.6 2412 No protein Retained intron - - - TSL:2,
PTPN6-222 ENST00000543744.1 2043 No protein Retained intron - - - TSL:1,
PTPN6-213 ENST00000539365.1 907 No protein Retained intron - - - TSL:5,
PTPN6-218 ENST00000542761.5 795 No protein Retained intron - - - TSL:2,
PTPN6-210 ENST00000538318.5 759 No protein Retained intron - - - TSL:5,
PTPN6-208 ENST00000536521.5 513 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PTPN6
This KO Strategy loading
Red Cotton™ Notes Gene PTPN6 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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