CRISPR-U™ Gene Knockout Cell Line
Strategy
PTPN6 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PTPN6 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PTPN6 |
| Gene id | 5777 |
| Organism | Homo sapiens |
| Official full symbol | protein tyrosine phosphatase non-receptor type 6 |
| Gene type | protein-coding |
| Also known as | HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L, SHP1 |
| Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PTPN6-204 | ENST00000456013.5 | 2389 | 624aa | Protein coding | CCDS44821 | P29350-4 | - | TSL:1, GENCODE basic, |
| PTPN6-201 | ENST00000318974.14 | 2161 | 595aa | Protein coding | CCDS44820 | P29350-1 | NM_002831.6 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| PTPN6-202 | ENST00000399448.5 | 2159 | 597aa | Protein coding | CCDS41744 | P29350-3 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| PTPN6-220 | ENST00000543115.5 | 650 | 130aa | Protein coding | - | F5H5H9 | - | CDS 3' incomplete, TSL:4, |
| PTPN6-215 | ENST00000541698.5 | 592 | 150aa | Protein coding | - | F5H1Z8 | - | CDS 3' incomplete, TSL:4, |
| PTPN6-211 | ENST00000538715.5 | 579 | 165aa | Protein coding | - | F5H0N8 | - | CDS 3' incomplete, TSL:4, |
| PTPN6-217 | ENST00000542462.1 | 570 | 144aa | Protein coding | - | F5GY79 | - | CDS 3' incomplete, TSL:4, |
| PTPN6-223 | ENST00000545153.5 | 959 | 72aa | Nonsense mediated decay | - | U3KQS1 | - | TSL:3, |
| PTPN6-219 | ENST00000542848.5 | 656 | 58aa | Nonsense mediated decay | - | F5H4Z1 | - | TSL:5, |
| PTPN6-207 | ENST00000536013.5 | 602 | 56aa | Nonsense mediated decay | - | F5H1V7 | - | TSL:5, |
| PTPN6-206 | ENST00000535462.5 | 543 | 60aa | Nonsense mediated decay | - | F5GXD4 | - | TSL:4, |
| PTPN6-212 | ENST00000539029.5 | 705 | No protein | Processed transcript | - | - | - | TSL:3, |
| PTPN6-209 | ENST00000537533.1 | 673 | No protein | Processed transcript | - | - | - | TSL:3, |
| PTPN6-216 | ENST00000542277.5 | 664 | No protein | Processed transcript | - | - | - | TSL:3, |
| PTPN6-214 | ENST00000540740.5 | 592 | No protein | Processed transcript | - | - | - | TSL:4, |
| PTPN6-221 | ENST00000543120.5 | 564 | No protein | Processed transcript | - | - | - | TSL:4, |
| PTPN6-205 | ENST00000534900.5 | 538 | No protein | Processed transcript | - | - | - | TSL:4, |
| PTPN6-203 | ENST00000416215.6 | 2412 | No protein | Retained intron | - | - | - | TSL:2, |
| PTPN6-222 | ENST00000543744.1 | 2043 | No protein | Retained intron | - | - | - | TSL:1, |
| PTPN6-213 | ENST00000539365.1 | 907 | No protein | Retained intron | - | - | - | TSL:5, |
| PTPN6-218 | ENST00000542761.5 | 795 | No protein | Retained intron | - | - | - | TSL:2, |
| PTPN6-210 | ENST00000538318.5 | 759 | No protein | Retained intron | - | - | - | TSL:5, |
| PTPN6-208 | ENST00000536521.5 | 513 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PTPN6 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PTPN6 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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