CRISPR-U™ Gene Knockout Cell Line
Strategy
PTPN1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PTPN1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PTPN1 |
| Gene id | 5770 |
| Organism | Homo sapiens |
| Official full symbol | protein tyrosine phosphatase non-receptor type 1 |
| Gene type | protein-coding |
| Also known as | PTP1B |
| Summary | The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 20 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PTPN1-201 | ENST00000371621.5 | 3979 | 435aa | Protein coding | CCDS13430 | A8K3M3 P18031 | NM_002827.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PTPN1-202 | ENST00000541713.5 | 3469 | 362aa | Protein coding | CCDS63309 | B4DSN5 | - | TSL:2, GENCODE basic, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PTPN1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PTPN1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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