CRISPR-U™ Gene Knockout Cell Line
Strategy
PTMA Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PTMA Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PTMA |
| Gene id | 5757 |
| Organism | Homo sapiens |
| Official full symbol | prothymosin alpha |
| Gene type | protein-coding |
| Also known as | TMSA |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PTMA-202 | ENST00000409115.8 | 1215 | 110aa | Protein coding | CCDS46541 | P06454-2 | NM_002823.5 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| PTMA-201 | ENST00000341369.11 | 1212 | 111aa | Protein coding | CCDS42833 | P06454-1 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| PTMA-204 | ENST00000409683.5 | 933 | 107aa | Protein coding | - | B8ZZQ6 | - | TSL:3, GENCODE basic, |
| PTMA-206 | ENST00000412128.1 | 863 | 148aa | Protein coding | - | H7C2N1 | - | CDS 5' incomplete, TSL:3, |
| PTMA-205 | ENST00000410064.5 | 814 | 136aa | Protein coding | - | B8ZZW7 | - | TSL:2, GENCODE basic, |
| PTMA-203 | ENST00000409321.5 | 735 | 131aa | Protein coding | - | B8ZZA1 | - | TSL:3, GENCODE basic, |
| PTMA-207 | ENST00000440384.1 | 222 | 74aa | Protein coding | - | H7C031 | - | CDS 5' and 3' incomplete, TSL:3, |
| PTMA-208 | ENST00000448874.5 | 1108 | 43aa | Nonsense mediated decay | - | F8WCE8 | - | TSL:2, |
| PTMA-209 | ENST00000466801.5 | 784 | No protein | Processed transcript | - | - | - | TSL:5, |
| PTMA-212 | ENST00000481928.1 | 1635 | No protein | Retained intron | - | - | - | TSL:3, |
| PTMA-211 | ENST00000468027.5 | 621 | No protein | Retained intron | - | - | - | TSL:1, |
| PTMA-210 | ENST00000467816.1 | 378 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PTMA |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PTMA had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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