CRISPR-U™ Gene Knockout Cell Line Strategy

PTMA Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PTMA Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PTMA
Gene id 5757
Organism Homo sapiens
Official full symbol prothymosin alpha
Gene type protein-coding
Also known as TMSA
Genomic regions Chromosome 2
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PTMA-202 ENST00000409115.8 1215 110aa Protein coding CCDS46541 P06454-2 NM_002823.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
PTMA-201 ENST00000341369.11 1212 111aa Protein coding CCDS42833 P06454-1 - TSL:1, GENCODE basic, APPRIS ALT1,
PTMA-204 ENST00000409683.5 933 107aa Protein coding - B8ZZQ6 - TSL:3, GENCODE basic,
PTMA-206 ENST00000412128.1 863 148aa Protein coding - H7C2N1 - CDS 5' incomplete, TSL:3,
PTMA-205 ENST00000410064.5 814 136aa Protein coding - B8ZZW7 - TSL:2, GENCODE basic,
PTMA-203 ENST00000409321.5 735 131aa Protein coding - B8ZZA1 - TSL:3, GENCODE basic,
PTMA-207 ENST00000440384.1 222 74aa Protein coding - H7C031 - CDS 5' and 3' incomplete, TSL:3,
PTMA-208 ENST00000448874.5 1108 43aa Nonsense mediated decay - F8WCE8 - TSL:2,
PTMA-209 ENST00000466801.5 784 No protein Processed transcript - - - TSL:5,
PTMA-212 ENST00000481928.1 1635 No protein Retained intron - - - TSL:3,
PTMA-211 ENST00000468027.5 621 No protein Retained intron - - - TSL:1,
PTMA-210 ENST00000467816.1 378 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PTMA
This KO Strategy loading
Red Cotton™ Notes Gene PTMA had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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