CRISPR-U™ Gene Knockout Cell Line
Strategy
NLGN4X Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NLGN4X Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NLGN4X |
| Gene id | 57502 |
| Organism | Homo sapiens |
| Official full symbol | neuroligin 4 X-linked |
| Gene type | protein-coding |
| Also known as | ASPGX2, AUTSX2, HLNX, HNL4X, NLGN4 |
| Summary | This gene encodes a member of the type-B carboxylesterase/lipase protein family. The encoded protein belongs to a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. The encoded protein interacts with discs large homolog 4 (DLG4). Mutations in this gene have been associated with autism and Asperger syndrome. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NLGN4X-203 | ENST00000381093.6 | 5865 | 816aa | Protein coding | CCDS14126 | Q8N0W4-1 | - | TSL:2, GENCODE basic, |
| NLGN4X-204 | ENST00000381095.8 | 5858 | 816aa | Protein coding | CCDS14126 | Q8N0W4-1 | NM_181332.3 | TSL:1, GENCODE basic, MANE Select v0.92, |
| NLGN4X-201 | ENST00000275857.10 | 5454 | 816aa | Protein coding | CCDS14126 | Q8N0W4-1 | - | TSL:1, GENCODE basic, |
| NLGN4X-202 | ENST00000381092.1 | 3750 | 816aa | Protein coding | CCDS14126 | Q8N0W4-1 | - | TSL:2, GENCODE basic, |
| NLGN4X-208 | ENST00000538097.6 | 3124 | 836aa | Protein coding | - | Q8N0W4-2 | - | TSL:1, GENCODE basic, APPRIS P1, |
| NLGN4X-205 | ENST00000469740.1 | 894 | 152aa | Protein coding | - | A0A2R8YCI5 | - | CDS 3' incomplete, TSL:3, |
| NLGN4X-207 | ENST00000483337.1 | 749 | 59aa | Protein coding | - | A0A2R8Y6F7 | - | CDS 3' incomplete, TSL:3, |
| NLGN4X-206 | ENST00000477079.1 | 600 | No protein | Processed transcript | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NLGN4X |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NLGN4X had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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