CRISPR-U™ Gene Knockout Cell Line Strategy

NLGN4X Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NLGN4X Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NLGN4X
Gene id 57502
Organism Homo sapiens
Official full symbol neuroligin 4 X-linked
Gene type protein-coding
Also known as ASPGX2, AUTSX2, HLNX, HNL4X, NLGN4
Summary This gene encodes a member of the type-B carboxylesterase/lipase protein family. The encoded protein belongs to a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. The encoded protein interacts with discs large homolog 4 (DLG4). Mutations in this gene have been associated with autism and Asperger syndrome. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome X
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NLGN4X-203 ENST00000381093.6 5865 816aa Protein coding CCDS14126 Q8N0W4-1 - TSL:2, GENCODE basic,
NLGN4X-204 ENST00000381095.8 5858 816aa Protein coding CCDS14126 Q8N0W4-1 NM_181332.3 TSL:1, GENCODE basic, MANE Select v0.92,
NLGN4X-201 ENST00000275857.10 5454 816aa Protein coding CCDS14126 Q8N0W4-1 - TSL:1, GENCODE basic,
NLGN4X-202 ENST00000381092.1 3750 816aa Protein coding CCDS14126 Q8N0W4-1 - TSL:2, GENCODE basic,
NLGN4X-208 ENST00000538097.6 3124 836aa Protein coding - Q8N0W4-2 - TSL:1, GENCODE basic, APPRIS P1,
NLGN4X-205 ENST00000469740.1 894 152aa Protein coding - A0A2R8YCI5 - CDS 3' incomplete, TSL:3,
NLGN4X-207 ENST00000483337.1 749 59aa Protein coding - A0A2R8Y6F7 - CDS 3' incomplete, TSL:3,
NLGN4X-206 ENST00000477079.1 600 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NLGN4X
This KO Strategy loading
Red Cotton™ Notes Gene NLGN4X had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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