CRISPR-U™ Gene Knockout Cell Line Strategy

PTGS2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PTGS2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PTGS2
Gene id 5743
Organism Homo sapiens
Official full symbol prostaglandin-endoperoxide synthase 2
Gene type protein-coding
Also known as COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2
Summary Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.
Genomic regions Chromosome 1
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PTGS2-205 ENST00000680451.1 4709 604aa Protein coding CCDS1371 - - GENCODE basic, APPRIS P1,
PTGS2-201 ENST00000367468.10 4510 604aa Protein coding CCDS1371 P35354 NM_000963.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PTGS2-206 ENST00000681605.1 4506 107aa Nonsense mediated decay - - - -
PTGS2-204 ENST00000559627.1 1705 163aa Nonsense mediated decay - Q6ZYK7 - TSL:1,
PTGS2-203 ENST00000490885.6 3690 No protein Retained intron - - - TSL:1,
PTGS2-202 ENST00000466691.1 888 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PTGS2
This KO Strategy loading
Red Cotton™ Notes Gene PTGS2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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