CRISPR-U™ Gene Knockout Cell Line
Strategy
PTGS2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PTGS2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PTGS2 |
| Gene id | 5743 |
| Organism | Homo sapiens |
| Official full symbol | prostaglandin-endoperoxide synthase 2 |
| Gene type | protein-coding |
| Also known as | COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2 |
| Summary | Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PTGS2-205 | ENST00000680451.1 | 4709 | 604aa | Protein coding | CCDS1371 | - | - | GENCODE basic, APPRIS P1, |
| PTGS2-201 | ENST00000367468.10 | 4510 | 604aa | Protein coding | CCDS1371 | P35354 | NM_000963.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PTGS2-206 | ENST00000681605.1 | 4506 | 107aa | Nonsense mediated decay | - | - | - | - |
| PTGS2-204 | ENST00000559627.1 | 1705 | 163aa | Nonsense mediated decay | - | Q6ZYK7 | - | TSL:1, |
| PTGS2-203 | ENST00000490885.6 | 3690 | No protein | Retained intron | - | - | - | TSL:1, |
| PTGS2-202 | ENST00000466691.1 | 888 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PTGS2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PTGS2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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