CRISPR-U™ Gene Knockout Cell Line
Strategy
CLK4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CLK4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CLK4 |
| Gene id | 57396 |
| Organism | Homo sapiens |
| Official full symbol | CDC like kinase 4 |
| Gene type | protein-coding |
| Summary | The protein encoded by this gene belongs to the CDC2-like protein kinase (CLK) family. This protein kinase can interact with and phosphorylate the serine- and arginine-rich (SR) proteins, which are known to play an important role in the formation of spliceosomes, and thus may be involved in the regulation of alternative splicing. Studies in the Israeli sand rat Psammomys obesus suggested that the ubiquitin-like 5 (UBL5/BEACON), a highly conserved ubiquitin-like protein, may interact with and regulate the activity of this kinase. Multiple alternatively spliced transcript variants have been observed, but the full-length natures of which have not yet been determined. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CLK4-201 | ENST00000316308.9 | 2504 | 481aa | Protein coding | CCDS4437 | Q9HAZ1 | NM_020666.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CLK4-215 | ENST00000611733.4 | 2182 | 373aa | Protein coding | - | A0A087WX74 | - | TSL:5, GENCODE basic, |
| CLK4-208 | ENST00000520957.1 | 1690 | 54aa | Protein coding | - | Q6P090 | - | TSL:1, GENCODE basic, |
| CLK4-214 | ENST00000611575.4 | 1206 | 373aa | Protein coding | - | B7ZL31 | - | TSL:1, GENCODE basic, |
| CLK4-209 | ENST00000521621.5 | 2780 | 53aa | Nonsense mediated decay | - | Q68D95 | - | TSL:1, |
| CLK4-210 | ENST00000522136.5 | 1955 | 143aa | Nonsense mediated decay | - | E7ES88 | - | TSL:1, |
| CLK4-211 | ENST00000522556.1 | 1249 | 89aa | Nonsense mediated decay | - | E7EWJ6 | - | TSL:1, |
| CLK4-212 | ENST00000522749.5 | 572 | No protein | Processed transcript | - | - | - | TSL:3, |
| CLK4-206 | ENST00000520878.5 | 3722 | No protein | Retained intron | - | - | - | TSL:1, |
| CLK4-203 | ENST00000519583.5 | 3462 | No protein | Retained intron | - | - | - | TSL:1, |
| CLK4-202 | ENST00000519132.5 | 3129 | No protein | Retained intron | - | - | - | TSL:1, |
| CLK4-204 | ENST00000520126.1 | 1316 | No protein | Retained intron | - | - | - | TSL:2, |
| CLK4-207 | ENST00000520909.5 | 1305 | No protein | Retained intron | - | - | - | TSL:1, |
| CLK4-205 | ENST00000520199.5 | 857 | No protein | Retained intron | - | - | - | TSL:2, |
| CLK4-213 | ENST00000523013.1 | 735 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CLK4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CLK4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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Work flow
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