CRISPR-U™ Gene Knockout Cell Line Strategy

CLK4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CLK4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CLK4
Gene id 57396
Organism Homo sapiens
Official full symbol CDC like kinase 4
Gene type protein-coding
Summary The protein encoded by this gene belongs to the CDC2-like protein kinase (CLK) family. This protein kinase can interact with and phosphorylate the serine- and arginine-rich (SR) proteins, which are known to play an important role in the formation of spliceosomes, and thus may be involved in the regulation of alternative splicing. Studies in the Israeli sand rat Psammomys obesus suggested that the ubiquitin-like 5 (UBL5/BEACON), a highly conserved ubiquitin-like protein, may interact with and regulate the activity of this kinase. Multiple alternatively spliced transcript variants have been observed, but the full-length natures of which have not yet been determined.
Genomic regions Chromosome 5
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CLK4-201 ENST00000316308.9 2504 481aa Protein coding CCDS4437 Q9HAZ1 NM_020666.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CLK4-215 ENST00000611733.4 2182 373aa Protein coding - A0A087WX74 - TSL:5, GENCODE basic,
CLK4-208 ENST00000520957.1 1690 54aa Protein coding - Q6P090 - TSL:1, GENCODE basic,
CLK4-214 ENST00000611575.4 1206 373aa Protein coding - B7ZL31 - TSL:1, GENCODE basic,
CLK4-209 ENST00000521621.5 2780 53aa Nonsense mediated decay - Q68D95 - TSL:1,
CLK4-210 ENST00000522136.5 1955 143aa Nonsense mediated decay - E7ES88 - TSL:1,
CLK4-211 ENST00000522556.1 1249 89aa Nonsense mediated decay - E7EWJ6 - TSL:1,
CLK4-212 ENST00000522749.5 572 No protein Processed transcript - - - TSL:3,
CLK4-206 ENST00000520878.5 3722 No protein Retained intron - - - TSL:1,
CLK4-203 ENST00000519583.5 3462 No protein Retained intron - - - TSL:1,
CLK4-202 ENST00000519132.5 3129 No protein Retained intron - - - TSL:1,
CLK4-204 ENST00000520126.1 1316 No protein Retained intron - - - TSL:2,
CLK4-207 ENST00000520909.5 1305 No protein Retained intron - - - TSL:1,
CLK4-205 ENST00000520199.5 857 No protein Retained intron - - - TSL:2,
CLK4-213 ENST00000523013.1 735 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CLK4
This KO Strategy loading
Red Cotton™ Notes Gene CLK4 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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